SIRPA antibody (AA 235-373)

Catalog No. ABIN5611267

Product Details anti-SIRPA Antibody

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Binding Specificity: AA 235-373

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Neutralization (Neut)

Purification: purified

Immunogen: Purified recombinant fragment of human CD172A (AA: extra 235-373) expressed in E. coli.

Clone: 2H7E2

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: N/A, FCM: 1:200 - 1:400, IHC: N/A

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-SIRPA antibody (ABIN5611267)

Deuse, Hu, Agbor-Enoh, Jang, Alawi, Saygi, Gravina, Tediashvili, Nguyen, Liu, Valantine, Lanier, Schrepfer: "The SIRPα-CD47 immune checkpoint in NK cells." in: The Journal of experimental medicine, Vol. 218, Issue 3, (2021) (PubMed).

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A (SIRPA Products)

Synonyms: BIT antibody, CD172A antibody, MFR antibody, MYD-1 antibody, P84 antibody, PTPNS1 antibody, SHPS1 antibody, SIRP antibody, SIRPA antibody, SIRP-A1 antibody, SIRP-B1 antibody, AI835480 antibody, Bit antibody, CD172a antibody, Ptpns1 antibody, SHP-1 antibody, SHPS-1 antibody, swc3 antibody, signal regulatory protein alpha antibody, myeloperoxidase antibody, signal-regulatory protein alpha antibody, tyrosine-protein phosphatase non-receptor type substrate 1 antibody, SIRPA antibody, MPO antibody, LOC458039 antibody, LOC100400378 antibody, LOC100590224 antibody, Sirpa antibody

Background:

Description: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.

Aliases: BIT, MFR, P84, SIRP, MYD-1, SHPS1, SIRPA, PTPNS1

Molecular Weight: 55 kDa

Gene ID: 140885

HGNC: 140885