Recognizes a (Mr 110 kDa) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).(1) This antibody blocks binding of HNK-1 (Leu. 7) antibody.(3) The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.
Characteristics
MAb to CD57, Monoclonal antibody to Human CD57/HNK-1 (Natural Killer cells)
B3GAT1
Reactivity: Human
FACS, IHC
Host: Mouse
Monoclonal
NK-1
SPRD
Application Notes
Suitable for use in flow cytometry, Western blotting and immunohistochemistry (formalin fixed paraffin sections). We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Short term (up to one month) store at 2-8 °C. Long term, aliquot and store at -28 °C.