CD57 antibody

Catalog No. ABIN5526903

The Mouse Monoclonal anti-CD57 antibody is suitable to detect CD57 in samples from Human. It has been validated for FACS, WB, IHC (p), ICC and IHC (fro).

Quick Overview for CD57 antibody (ABIN5526903)

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TB01

Product Details anti-CD57 Antibody

Purpose: Anti-Hu CD57 Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: A pool of neuroblastoma cell lines

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Alternative Name: CD57

Background: Beta-1,3-glucuronyltransferase 1,CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.,HNK1, LEU7, 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc

Gene ID: 27087

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process