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CD69 antibody (CD69)

Details for Product anti-CD69 Antibody No. ABIN263925, Supplier: Login to see
Antigen
  • 5830438K24Rik
  • AI452015
  • AIM
  • VEA
  • BL-AC/P26
  • CLEC2C
  • EA1
  • GP32/28
  • MLR-3
Reactivity
Human
213
150
28
5
5
5
5
5
Host
Mouse
146
104
74
20
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is un-conjugated
50
40
21
18
11
9
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
260
84
54
43
40
24
21
18
12
10
9
5
4
3
2
2
2
2
2
1
Supplier
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Immunogen Anti-µ-stimulated human B lymphocytes
Clone FN50
Isotype IgG1
Specificity The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.
Characteristics Synonyms: GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM, Early T-cell activation antigen p60, Earlyactivation antigen CD69, Activation inducer molecule, C-type lectin domain family 2member C
Purification Affinity Chromatography on Protein A
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.Synonyms: AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leu-23, MLR-3
Gene ID 969
UniProt Q07108
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology
Pathways
Application Notes Flow Cytometry: 5 μg/mL. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together withTPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 tostimulated peripheral blood B cells has similar, but weaker effect.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS with 15 mM Sodium Azide, pH ~7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD69 antibody (CD69) (ABIN263925) anti-CD69 (CD69) antibody
Product cited in: Hrdinka, Dráber, Stepánek et al.: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Tomescu, Chehimi, Maino et al.: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Background publications Pitsios, Dimitrakopoulou, Tsalimalma et al.: "Expression of CD69 on T-cell subsets in HIV-1 disease." in: Scandinavian journal of clinical and laboratory investigation, Vol. 68, Issue 3, pp. 233-41, 2008 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Nielsen, Afzelius, Ersbøll et al.: "Expression of the activation antigen CD69 predicts functionality of in vitro expanded peripheral blood mononuclear cells (PBMC) from healthy donors and HIV-infected patients." in: Clinical and experimental immunology, Vol. 114, Issue 1, pp. 66-72, 1998 (PubMed).

López-Cabrera, Santis, Fernández-Ruiz et al.: "Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 537-47, 1993 (PubMed).