CD69 antibody (CD69) (FITC)

Details for Product anti-CD69 Antibody No. ABIN302016
Antigen
  • 5830438K24Rik
  • AI452015
  • AIM
  • VEA
  • BL-AC/P26
  • CLEC2C
  • EA1
  • GP32/28
  • MLR-3
  • Cd69 molecule
  • CD69 antigen
  • CD69 molecule
  • Cd69
  • CD69
Reactivity
Human
198
142
44
8
7
7
7
5
4
2
1
Host
Mouse
118
88
82
15
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is conjugated to FITC
45
41
22
15
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
228
86
56
54
48
28
16
13
13
13
6
4
4
3
1
1
Options
Immunogen anti-µ,-stimulated human B lymphocytes
Clone FN50
Isotype IgG1
Specificity The antibody FN50 recognizes CD69, an lymphocyte early activation marker.
HLDA IV, WS Code A 91
Cross-Reactivity (Details) Human, Other not determined
Antigen
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD69 (CD69) antibody (FITC) (ABIN302016) Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) afte...
Flow Cytometry (FACS) image for anti-CD69 (CD69) antibody (FITC) (ABIN302016) Surface staining of human PHA-activated peripheral blood using anti-CD69 antibody (cl...
Product cited in: Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Background publications Pitsios, Dimitrakopoulou, Tsalimalma, Kordossis, Choremi-Papadopoulou: "Expression of CD69 on T-cell subsets in HIV-1 disease." in: Scandinavian journal of clinical and laboratory investigation, Vol. 68, Issue 3, pp. 233-41, 2008 (PubMed).

Konjevi?, Jovi?, Vuleti?, Radulovi?, Jeli?, Spuzi?: "CD69 on CD56+ NK cells and response to chemoimmunotherapy in metastatic melanoma." in: European journal of clinical investigation, Vol. 37, Issue 11, pp. 887-96, 2007 (PubMed).