CD69 antibody (CD69) (FITC)

Details for Product anti-CD69 Antibody No. ABIN135255
Antigen
  • 5830438K24Rik
  • AI452015
  • AIM
  • VEA
  • BL-AC/P26
  • CLEC2C
  • EA1
  • GP32/28
  • MLR-3
  • Cd69 molecule
  • CD69 antigen
  • CD69 molecule
  • Cd69
  • CD69
Reactivity
Mouse (Murine)
191
134
43
8
7
7
7
5
4
2
1
Host
Hamster
112
87
75
15
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is conjugated to FITC
43
38
20
13
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
213
86
58
53
47
30
17
15
13
13
6
6
5
3
1
1
Options
Immunogen Mouse dendritic epidermal cell line Y245
Clone H1-2F3
Isotype IgG
Specificity Mouse CD69, Mr 85 kDa (unreduced)
Characteristics Hamster Anti-Mouse CD69-FITC
Purification Protein A
Antigen
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.
Application Notes
  • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Costim - Reported in literature , Activ - Reported in literature
  • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and PACBLU conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Comment

In vitro T cell and NK-cell activation

Sample Volume 1 mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 0.5 mg in 1.0 mL of PBS/Sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Supplier Images
Image no. 1 for anti-CD69 (CD69) antibody (FITC) (ABIN135255) Con-A stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-F...
Background publications Karlhofer, Yokoyama: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, 1991 (PubMed).

Yokoyama, Koning, Kehn, Pereira, Stingl, Coligan, Shevach: "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 369-76, 1988 (PubMed).