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CPE antibody (AA 42-453)

CPE Reactivity: Human WB, ELISA, IP, FACS Host: Goat Polyclonal unconjugated

Catalog No. ABIN299519

Datasheet as PDF

Target See all CPE Antibodies

CPE (Carboxypeptidase E (CPE))
Binding Specificity
15

5

5

3

2

2

2

2

2

1

1

1

1

1

1

1
AA 42-453
Reactivity
52

34

32

2

2

2

2

1

1

1
Human
Host
51

1
Goat
Compatible Secondaries
Clonality
51

1
Polyclonal
Conjugate
22

4

4

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CPE antibody is un-conjugated
Application
37

18

13

13

10

8

6

4

4

4

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity

Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.

Purification

Affinity purified

Immunogen

NS0-derived recombinant human Carboxypeptidase E (aa42-453).

Isotype

IgG

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CPE Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

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CPE Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Carboxypeptidase E (CPE) (AA 271-320) antibody
CPE Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Sterile PBS

Concentration

Lot specific

Buffer

Lyophilized from PBS, 5 % trehalose.

Handling Advice

Avoid freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
Target

CPE (Carboxypeptidase E (CPE))

Alternative Name

Carboxypeptidase e / CPE (CPE Products)

Background

Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase

Gene ID

1363

UniProt

P16870

Pathways

Peptide Hormone Metabolism, Synaptic Membrane