Transferrin Receptor antibody

Catalog No. ABIN316908

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody recognises CD71, a 95 kDa cell surface protein that is otherwise known as transferrin receptor. This antibody recognises native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the Mouse.

Characteristics: Synonyms: TfR1, p90, Transferrin receptor protein 1

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin Level: < 0.01 Eu/μg

Immunogen: Mouse transformed endothelioma cell line t. end1. Spleen cells from immunised Lewis rats were fused with the cells of the mouse NSOmyeloma cell line

Clone: 8D3

Isotype: IgG2a

Application Details

Application Notes: Functional Assays. Flow Cytometry: Use 10 μL of 1/100-1/200 diluetd CD71 antibody to label 10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Endotoxin Level: <0.01 Eu/μg

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC (TFRC Products)

Background: CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 22042

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis