Transferrin Receptor antibody
-
- Target See all Transferrin Receptor (TFRC) Antibodies
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
-
Reactivity
- Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This Transferrin Receptor antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody reacts with the Transferrin Receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90 kDa by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).
- Purification
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Immunogen
- Mouse Concanavilin A activated spleen cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Clone
- YTA74-4
- Isotype
- IgG2a
- Top Product
- Discover our top product TFRC Primary Antibody
-
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC Reactivity: Human FACS, IP, ICC Host: Mouse Monoclonal MEM-75 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibodyTFRC Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibodyTFRC Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibodyTFRC Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (Biotin)TFRC Reactivity: Human FACS, IP, ICC Host: Mouse Monoclonal MEM-75 Biotin
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 649-677), (C-Term) antibodyTFRC Reactivity: Human FACS, WB, IHC (p) Host: Rabbit Polyclonal RB20736 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibodyTFRC Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1A1B2 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibodyTFRC Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1E6 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 567-744) antibodyTFRC Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 572-757) antibodyTFRC Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
-
- Application Notes
-
Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- Alternative Name
- CD71 / TFRC (TFRC Products)
- Background
- CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.Synonyms: TfR1, Transferrin receptor protein 1, p90
- Gene ID
- 22042
- NCBI Accession
- NP_035768
- UniProt
- Q62351
- Pathways
- Transition Metal Ion Homeostasis
-
