KRT14 antibody (C-Term)

Catalog No. ABIN319880

Product Details anti-KRT14 Antibody

Target: KRT14 (Keratin 14 (KRT14))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.

Purification: Ion Exchange Chromatography

Immunogen: Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin.

Clone: LL002

Isotype: IgG3

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/100 diluted antibody to label 10^6 cells in 100 μL (Membrane permeabilisation is required). Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/200): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer is recommended for this purpose, Enzyme digestion is not recommended. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14 (KRT14 Products)

Background: Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Gene ID: 3861

NCBI Accession: NP_000517

UniProt: P02533