Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) (Internal Region) antibody

Details for Product No. ABIN334426
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Antigen
Synonyms AIF4, AIP4, NAPP1, dJ468O1.1, 6720481N21Rik, 8030492O04Rik, A130065M08, C230047C07Rik
Epitope
Internal Region
(13), (10), (8), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(42), (21), (1)
Host
Goat
(37), (8), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(34), (30), (9), (4), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334426
374.27 $
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Immunogen Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ITCH / AIF4
Background Synonyms: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiquitin protein ligase ITCH
Gene ID 83737, 16396, 311567
NCBI Accession NP_113671
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7 kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) (Internal Region) antibody ABIN334426 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Di Marcotullio, Ferretti, Greco et al.: "Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination." in: Nature cell biology, Vol. 8, Issue 12, pp. 1415-23, 2006 (PubMed).

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