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Details for Product No. ABIN334426

Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) (Internal Region) antibody

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Antigen
Synonyms AIF4, AIP4, NAPP1, dJ468O1.1
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN334426
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Shipping to
Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ITCH / AIF4
Background Synonyms: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiquitin protein ligase ITCH
Gene ID 83737, 16396, 311567
NCBI Accession NP_113671.3
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7 kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Di Marcotullio, Ferretti, Greco et al.: "Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination." in: Nature cell biology, Vol. 8, Issue 12, pp. 1415-23, 2006 (PubMed).

Alternatives for antigen "Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH)", type "Antibodies"
Hosts (20), (5), (2)
Reactivities (22), (13), (1), (1)
Applications (20), (18), (5), (1), (1), (1)
Epitopes (5), (4), (3), (2), (1), (1), (1), (1), (1)
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