ITCH antibody (AA 143-192)

Catalog No. ABIN6743170

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: AA 143-192

Reactivity: Human, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ITCH

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Rabbit (92%) Bovine, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human ITCH (NP_113671). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Elephant, Dog, Rabbit, Guinea pig (92%), Galago, Bovine, Bat, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Synonyms: AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody

Background: Name/Gene ID: ITCH

Synonyms: ITCH, AIF4, AIP4, DJ468O1.1, NAPP1, NFE2-associated polypeptide 1

Gene ID: 83737

NCBI Accession: NP_113671

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events