Cytokeratin 18 antibody (FITC)
-
- Target See all Cytokeratin 18 (KRT18) Antibodies
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Cytokeratin 18 antibody is conjugated to FITC
-
Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Molecular Weight: 45 kDa.
- Immunogen
- Human breast cancer PMC 42 cells
- Clone
- CK209
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT18 Primary Antibody
-
-
- Application Notes
- IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears). For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (Antigen retriever like citrate enhances the staining of FFPE tissue sections) (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Human skin, breast, lung ca.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Cytokeratins are intermediate filament keratins found in
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
- Alternative Name
- Cytokeratin 18 (KRT18 Products)
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
-