SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody

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Synonyms ARX, SAE2, Sae2, Uble1b, ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, sae2, sae2-B, uba2-a, uba2-b, uble1b-B
(9), (3), (1), (1), (1), (1), (1)
(35), (7), (4), (1), (1)
(31), (4), (1)
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(31), (20), (12), (11), (2), (1)
Pubmed 3 references available
Catalog no. ABIN356597
Quantity 0.4 mL
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBA2.
Specificity This antibody is specific to UBA2 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE2 / UBA2
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N- terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C- terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
Alternate names: ARX, Anthracycline-associated resistance ARX, HRIHFB2115, SUMO-activating enzyme subunit 2, UBLE1B, Ubiquitin-like 1-activating enzyme E1B
Gene ID 10054
NCBI Accession 9606
UniProt Q9UBT2
Research Area Proteolysis / Ubiquitin
Application Notes ELISA: 1/1,000. Western blot: 1/100~1/500. Immunohistochemistry: 1/50~1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody (2) anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody (Image 2)
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Hosts (31), (4), (1)
Reactivities (35), (7), (4), (1), (1)
Applications (31), (20), (12), (11), (2), (1)
Epitopes (9), (3), (1), (1), (1), (1), (1)
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