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SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody

Details for Product No. ABIN356597, Supplier: Log in to see
Antigen
  • ATSAE2
  • EMB2764
  • EMBRYO DEFECTIVE 2764
  • F3K23.23
  • F3K23_23
  • SUMO-ACTIVATING ENZYME 2
  • SUMO-activating enzyme 2
  • ARX
  • SAE2
  • AA986091
  • Arx
  • Sae2
  • UBA1
  • Ubl1a2
  • Uble1b
  • sae2
  • sae2-B
  • uba2-a
  • uba2-b
  • uble1b-B
  • sae2b
  • uble1b
  • wu:fi17g06
  • zgc:66354
Epitope
C-Term
32
7
6
3
2
1
1
1
1
1
1
1
Reactivity
Human
69
10
4
1
1
Host
Rabbit
60
5
5
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
61
37
29
17
14
3
2
2
2
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBA2.
Isotype Ig
Specificity This antibody is specific to UBA2 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE2 / UBA2 (UBA2 Antibody Abstract)
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: ARX, Anthracycline-associated resistance ARX, HRIHFB2115, SUMO-activating enzyme subunit 2, UBLE1B, Ubiquitin-like 1-activating enzyme E1B
Gene ID 10054, 9606
UniProt Q9UBT2
Research Area Proteolysis / Ubiquitin
Pathways
Application Notes ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody (ABIN356597) anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody
 image for anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody (ABIN356597) anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term) antibody (Image 2)
Background publications Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).