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RAF1 antibody (pSer471)

RAF1 Reactivity: Human EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN358302
  • Target See all RAF1 Antibodies
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Binding Specificity
    • 49
    • 43
    • 35
    • 34
    • 32
    • 27
    • 23
    • 23
    • 23
    • 22
    • 19
    • 18
    • 15
    • 13
    • 13
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer471
    Reactivity
    • 447
    • 231
    • 159
    • 14
    • 13
    • 12
    • 12
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 478
    • 15
    • 2
    • 2
    Rabbit
    Clonality
    • 453
    • 44
    Polyclonal
    Conjugate
    • 202
    • 37
    • 28
    • 27
    • 20
    • 20
    • 20
    • 20
    • 20
    • 20
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    This RAF1 antibody is un-conjugated
    Application
    • 298
    • 217
    • 105
    • 104
    • 89
    • 75
    • 49
    • 31
    • 30
    • 24
    • 15
    • 11
    • 10
    • 3
    • 2
    • 2
    • 1
    Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects RAF1 pSer471. Predicted to cross react with Mouse (100 % Antigen Homology).
    Purification
    Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
    Immunogen
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S471 of human RAF1.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product RAF1 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Dot Blot: 1/100-1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Alternative Name
    RAF1 (RAF1 Products)
    Synonyms
    CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
    Background
    Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
    Molecular Weight
    72921 Da
    Gene ID
    5894, 9606
    UniProt
    P04049
    Pathways
    MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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