phosphorylase Kinase, gamma 2 (Testis) (PHKG2) (N-Term) antibody

Details for Product No. ABIN359432
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Antigen
Synonyms PHKG2, MGC145608, zgc:55863, GSD9C, 1500017I02Rik
Epitope
N-Term
(18), (9), (7), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(50), (20), (16), (13), (13), (13), (1), (1), (1)
Host
Rabbit
(41), (11)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(40), (24), (20), (10), (7), (5), (3), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN359432
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PHKG2.
Specificity This antibody detects PHKG2 at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PHKG2
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).
Alternate names: PHK-gamma-T, PSK-C3, Phosphorylase b kinase gamma catalytic chain testis/liver isoform, Phosphorylase kinase subunit gamma-2
Molecular Weight 46442 Da
Gene ID 5261, 9606
UniProt P15735
Research Area Cancer, Metabolism
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Hanks: "Messenger ribonucleic acid encoding an apparent isoform of phosphorylase kinase catalytic subunit is abundant in the adult testis." in: Molecular endocrinology (Baltimore, Md.), Vol. 3, Issue 1, pp. 110-6, 1989 (PubMed).

Hanks: "Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 2, pp. 388-92, 1987 (PubMed).

Whitmore, Apostolou, Lane et al.: "Isolation and characterization of transcribed sequences from a chromosome 16 hn-cDNA library and the physical mapping of genes and transcribed sequences using a high-resolution somatic cell panel of human chromosome 16." in: Genomics, Vol. 20, Issue 2, pp. 169-75, 1994 (PubMed).

Maichele, Burwinkel, Maire et al.: "Mutations in the testis/liver isoform of the phosphorylase kinase gamma subunit (PHKG2) cause autosomal liver glycogenosis in the gsd rat and in humans." in: Nature genetics, Vol. 14, Issue 3, pp. 337-40, 1996 (PubMed).

Burwinkel, Shiomi, Al Zaben et al.: "Liver glycogenosis due to phosphorylase kinase deficiency: PHKG2 gene structure and mutations associated with cirrhosis." in: Human molecular genetics, Vol. 7, Issue 1, pp. 149-54, 1998 (PubMed).

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