Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Antigen: Lysosomal-Associated Membrane Protein 1 (LAMP1)

Synonyms: LAMPA, CD107a, LGP120

Clonality: Monoclonal (Ly1C6)

Host: Mouse

Reactivity: Rat (Rattus), Hamster

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN361797

Additional Information

Characteristics: Detects an ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.

Alternative name: LAMP1

Gene ID: 25328

Swiss-Prot: P14562

Immunogen: Rat liver lysosomal membrane preparations

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: Ly1C6

Description: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology . They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D , which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation.
Synonyms: CD107, CD107a, LAMPA, LGP120, IgpA

Sensitivity: 1µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.

Application Details

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cancer, Hematopoietic Progenitors, Organelles

Restrictions: For Research Use only

Images

Lamp1 (Ly1C6), IF in transfected HeLa cells. Courtesy of Robert H Edwards, U. of Cali, San Fran School of Medicine