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Details for Product No. ABIN371639

Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody (Sepharose)

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Antigen
Synonyms MORT1, Mort1/FADD, Mort1, TNFRSF6
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Sepharose
Application
»Alternatives ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN371639
Quantity 50 tests
Price
478.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days

Product details

Target Details Application Details Handling References Alternatives back to top
Clone 1A11
Isotype IgG1
Characteristics Synonyms: FAS-associated death domain protein, MORT1, GIG3, FAS-associating deathdomain-containing protein, Mediator of receptor induced toxicity, Growth-inhibiting gene 3protein

Target Details

Application Details Handling References Alternatives back to top
Alternative Name FADD
Background Fas-associated death domain (FADD, also known as MORT1) is an important apoptosisadapter molecule involved in the Fas-mediated cell death pathway. FADD associates withthe intracellular domain of FAS (CD95/APO-1) through their respective C-terminal deathdomains (DD). The N-terminal region of FADD contains a death effectors domain (DED)which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequentactivation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated(ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosiswhich is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival andproliferation. (Ref. 1-6)
UniProt Q13158
Research Area Cancer, Apoptosis/Necrosis

Application Details

Target Details Handling References Alternatives back to top
Application Notes ELISA. Immunoprecipitation. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Target Details Application Details References Alternatives back to top
Format Liquid
Buffer 2 ml of 100 mM Borate buffered saline, pH 8.2.No preservatives or amine-containing buffer salts added
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Store protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months

References

Target Details Application Details Handling Alternatives back to top
Background publications Boldin, Varfolomeev, Pancer et al.: "A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain." in: The Journal of biological chemistry, Vol. 270, Issue 14, pp. 7795-8, 1995 (PubMed).

Cleveland, Ihle: "Contenders in FasL/TNF death signaling." in: Cell, Vol. 81, Issue 4, pp. 479-82, 1995 (PubMed).

Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Newton, Harris, Bath et al.: "A dominant interfering mutant of FADD/MORT1 enhances deletion of autoreactive thymocytes and inhibits proliferation of mature T lymphocytes." in: The EMBO journal, Vol. 17, Issue 3, pp. 706-18, 1998 (PubMed).

Wieder, Essmann, Prokop et al.: "Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3." in: Blood, Vol. 97, Issue 5, pp. 1378-87, 2001 (PubMed).

Alternatives

Target Details Application Details Handling References back to top
Alternatives for antigen "Fas (TNFRSF6)-Associated Via Death Domain (FADD)", type "Antibodies"
Hosts (107), (37)
Reactivities (113), (47), (30), (12), (2), (1)
Applications (100), (67), (40), (29), (24), (19), (8), (4), (2), (2), (1), (1), (1)
Conjugates (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2)
Epitopes (29), (20), (10), (7), (6), (4), (1), (1)
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