FADD antibody (Sepharose)

Catalog No. ABIN371639

Product Details anti-FADD Antibody (Sepharose)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FADD antibody is conjugated to Sepharose

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for FADD.

Characteristics: Synonyms: FAS-associated death domain protein, MORT1, GIG3, FAS-associating deathdomain-containing protein, Mediator of receptor induced toxicity, Growth-inhibiting gene 3protein

Clone: 1A11

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 2 mLof 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Store protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD (FADD Products)

Background: Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (Ref.1-6)Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity

Gene ID: 8772

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades