Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody (Sepharose)

Details for Product No. ABIN371639
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Antigen
Synonyms MORT1, Mort1/FADD, Mort1, TNFRSF6
Reactivity
Human
(162), (73), (34), (12), (4), (3), (1)
Host
Mouse
(164), (55), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Sepharose
(7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3)
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(162), (98), (58), (40), (28), (18), (14), (8), (5), (4), (4), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 50 tests
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN371639
489.50 $
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Clone 1A11
Isotype IgG1
Specificity This antibody is specific for FADD.
Characteristics Synonyms: FAS-associated death domain protein, MORT1, GIG3, FAS-associating deathdomain-containing protein, Mediator of receptor induced toxicity, Growth-inhibiting gene 3protein
Alternative Name FADD
Background Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (Ref.1-6)Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity
Gene ID 8772
UniProt Q13158
Research Area Cancer, Apoptosis/Necrosis
Application Notes ELISA. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer 2 mLof 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Store protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Boldin, Varfolomeev, Pancer et al.: "A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain." in: The Journal of biological chemistry, Vol. 270, Issue 14, pp. 7795-8, 1995 (PubMed).

Cleveland, Ihle: "Contenders in FasL/TNF death signaling." in: Cell, Vol. 81, Issue 4, pp. 479-82, 1995 (PubMed).

Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Newton, Harris, Bath et al.: "A dominant interfering mutant of FADD/MORT1 enhances deletion of autoreactive thymocytes and inhibits proliferation of mature T lymphocytes." in: The EMBO journal, Vol. 17, Issue 3, pp. 706-18, 1998 (PubMed).

Wieder, Essmann, Prokop et al.: "Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3." in: Blood, Vol. 97, Issue 5, pp. 1378-87, 2001 (PubMed).

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