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BID antibody (BH3 Interacting Domain Death Agonist)

Details for Product anti-BID Antibody No. ABIN371640, Supplier: Login to see New
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Synonyms BID, bid, si:ch211-238n5.6, fp497, xbid, DKFZp469E066, FP497, 2700049M22Rik, AI875481, AU022477
(283), (133), (56), (2), (1)
(280), (69), (22), (10)
Clonality (Clone)
Monoclonal ()
This BID antibody is un-conjugated
(20), (18), (13), (8), (7), (7), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(292), (170), (109), (66), (37), (35), (28), (20), (13), (11), (10), (3), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Clone SB50a
Isotype IgG2b
Specificity This antibody is specific for Bid.
Characteristics Synonyms: p22 BID, BH3-interacting domain death agonist
Purification Purified
Alternative Name BID (BID Antibody Abstract)
Background Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF-alpha apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c. (Ref.1-10)Synonyms: BH3-interacting domain death agonist, p22 BID
Gene ID 637
UniProt P55957
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Cirman, Oresić, Mazovec et al.: "Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3578-87, 2004 (PubMed).

Chen, He, Zhan et al.: "Bid is cleaved by calpain to an active fragment in vitro and during myocardial ischemia/reperfusion." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 30724-8, 2001 (PubMed).

Stoka, Turk, Schendel et al.: "Lysosomal protease pathways to apoptosis. Cleavage of bid, not pro-caspases, is the most likely route." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3149-57, 2001 (PubMed).

Barry, Heibein, Pinkoski et al.: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, 2000 (PubMed).

Kudla, Montessuit, Eskes et al.: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).

Eskes, Desagher, Antonsson et al.: "Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane." in: Molecular and cellular biology, Vol. 20, Issue 3, pp. 929-35, 2000 (PubMed).

Gross, Yin, Wang et al.: "Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 1156-63, 1999 (PubMed).

Li, Zhu, Xu et al.: "Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis." in: Cell, Vol. 94, Issue 4, pp. 491-501, 1998 (PubMed).

Luo, Budihardjo, Zou et al.: "Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors." in: Cell, Vol. 94, Issue 4, pp. 481-90, 1998 (PubMed).

Wang, Yin, Chao et al.: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).

Catalog No. ABIN371640
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