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Details for Product No. ABIN371640

BH3 Interacting Domain Death Agonist (BID) antibody

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Synonyms FP497, AI875481, AU022477, 2700049M22Rik, BID, bid, si:ch211-238n5.6, fp497, xbid, DKFZp469E066
»Alternatives Human
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 10 references available
Catalog no. ABIN371640
Quantity 0.1 mg
313.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Clone SB50a
Isotype IgG2b
Characteristics Synonyms: p22 BID, BH3-interacting domain death agonist
Purification Purified
Alternative Name BID
Background Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or severalof the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member orBH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of livingcells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 inthe Fas and TNF-alpha apoptotic signaling pathways. Recent studies have suggested thatBid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. CleavedBid translocates to mitochondria where it induces the release of cytochrome c from theintermembrane space of the mitochondria. The release of cytochrome c initiates apoptosisby activating downstream caspases through binding to Apaf-1. Bid has also been shown tointeract with with Bax, another Bcl-2 family protein, to trigger a conformational change inBax leading to the dimerization and integration of Bax into the outer mitochondrialmembrane and activating the release of cytochrome c. (Ref. 1-10)
UniProt P55957
Research Area Cancer
Application Notes ELISA. Immunoprecipitation. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2.No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Wang, Yin, Chao et al.: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).

Luo, Budihardjo, Zou et al.: "Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors." in: Cell, Vol. 94, Issue 4, pp. 481-90, 1998 (PubMed).

Li, Zhu, Xu et al.: "Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis." in: Cell, Vol. 94, Issue 4, pp. 491-501, 1998 (PubMed).

Gross, Yin, Wang et al.: "Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 1156-63, 1999 (PubMed).

Eskes, Desagher, Antonsson et al.: "Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane." in: Molecular and cellular biology, Vol. 20, Issue 3, pp. 929-35, 2000 (PubMed).

Kudla, Montessuit, Eskes et al.: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).

Barry, Heibein, Pinkoski et al.: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, 2000 (PubMed).

Stoka, Turk, Schendel et al.: "Lysosomal protease pathways to apoptosis. Cleavage of bid, not pro-caspases, is the most likely route." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3149-57, 2001 (PubMed).

Chen, He, Zhan et al.: "Bid is cleaved by calpain to an active fragment in vitro and during myocardial ischemia/reperfusion." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 30724-8, 2001 (PubMed).

Cirman, Oresić, Mazovec et al.: "Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3578-87, 2004 (PubMed).

Alternatives for antigen "BH3 Interacting Domain Death Agonist (BID)", type "Antibodies"
Hosts (182), (37), (9), (6)
Reactivities (165), (109), (55), (4), (3)
Applications (188), (108), (65), (49), (40), (35), (15), (11), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1)
Conjugates (10), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2)
Epitopes (23), (19), (17), (16), (13), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)