BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195)

Details for Product anti-BID Antibody No. ABIN3042321
Antigen
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Alternatives
anti-Human BID antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 1-195
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34
31
18
15
15
11
9
9
8
7
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
318
159
79
5
3
3
2
Host
Rabbit
311
76
21
6
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
26
21
13
8
7
7
6
6
6
6
6
6
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
329
166
152
76
47
41
29
20
15
11
9
9
3
2
2
1
1
1
1
1
Options
Purpose Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(BID) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Immunogen E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(BID) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: BH3 interacting domain death agonist
Protein Name: BH3-interacting domain death agonist
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others BID products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name BID (BID Antibody Abstract)
Background BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Synonyms: Apoptic death agonist antibody|Apoptotic death agonist antibody|Apoptotic death agonist BID antibody|BH3 interacting domain death agonist antibody|BH3 interacting domain death agonist p11 antibody|BH3 interacting domain death agonist p13 antibody|BH3 interacting domain death agonist p15 antibody|BH3-interacting domain death agonist p11 antibody|BID antibody|BID isoform ES(1b) antibody|BID isoform L(2) antibody|BID isoform Si6 antibody|BID_HUMAN antibody|Desmocollin type 4 antibody|FP497 antibody|Human BID coding sequence antibody|MGC15319 antibody|MGC42355 antibody|p11 BID antibody|p13 BID antibody|p15 BID antibody|p22 BID antibody
Gene ID 637
UniProt P55957
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Bid is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Immunohistochemistry (IHC) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) Anti-Bid Picoband antibody, ICC: A549 Cell
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Rat Intestine Tissue
Flow Cytometry (FACS) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) Flow Cytometry analysis of MCF-7 cells using anti-BID antibody . Overlay histogram sh...
Product cited in: Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, 2016 (PubMed).

Wang, Gong, Chen, Xiong, Zhou, Huang, Kong: "NLRP3 inflammasome sequential changes in Staphylococcus aureus-induced mouse model of acute rhinosinusitis." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15806-20, 2015 (PubMed).

Li, Chen, Zhang, Song, Mu: "Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats." in: Neural regeneration research, Vol. 7, Issue 5, pp. 325-31, 2015 (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, 2013 (PubMed).

Yao, Peng, Peng, Tan, Wu, Wu, Chen, Li, Li, Zhu: "Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye." in: International journal of ophthalmology, Vol. 3, Issue 2, pp. 114-9, 2012 (PubMed).

Wang, Liu, Mi, Wang, Tang, Zhao, Li: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, 2010 (PubMed).

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