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Details for Product No. ABIN371647

Toll-Like Receptor 4 (TLR4) antibody

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Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 10 references available
Catalog no. ABIN371647
Quantity 0.1 mg
Price
275.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Clone 1E11
Isotype IgG2a
Characteristics Synonyms: Toll-like receptor 4
Purification Purified
Alternative Name CD284 / TLR4
Background The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteinscharacterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains aconserved region call the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRsrecognize specific molecular patterns that are present in microbial components andrespond to these components in order eliminate or limit invading microbes. To date, tenmammalian homologs of TLRs have been described. TLR4 is involved in the recognition oflipopolysaccharide (LPS), an abundant glycolipid of Gram-negative bacteria. Through itsassociation with MD-2 and CD14, TLR4 recognizes and initiates multiple intracellularsignaling events such as the activation of NF-kB leading to the ultimate synthesis andrelease of a number of proinflammatory mediators including interleukin-1 (IL-1),interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-alpha). TLR4 hasalso been associated in the recognition of and lipocheic acid(LTA), Hsp60 and Hsp70. (Ref. 1-10)
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Application Notes ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2.No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Zhang, Kirschning, Mancinelli et al.: "Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7611-4, 1999 (PubMed).

Chow, Young, Golenbock et al.: "Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 10689-92, 1999 (PubMed).

Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Ohashi, Burkart, Flohé et al.: "Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 558-61, 2000 (PubMed).

Lien, Means, Heine et al.: "Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide." in: The Journal of clinical investigation, Vol. 105, Issue 4, pp. 497-504, 2000 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Dziarski, Gupta: "Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes." in: Journal of endotoxin research, Vol. 6, Issue 5, pp. 401-5, 2001 (PubMed).

Habich, Baumgart, Kolb et al.: "The receptor for heat shock protein 60 on macrophages is saturable, specific, and distinct from receptors for other heat shock proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 569-76, 2002 (PubMed).

Asea, Rehli, Kabingu et al.: "Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15028-34, 2002 (PubMed).

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts (140), (127), (20), (5)
Reactivities (222), (89), (35), (25), (24), (21), (19), (12), (4), (3), (2), (2), (1), (1), (1)
Applications (150), (141), (63), (62), (43), (32), (29), (18), (17), (10), (9), (7), (6), (3), (2), (1), (1), (1), (1)
Conjugates (30), (28), (18), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (23), (13), (9), (9), (7), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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