Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody

Details for Product No. ABIN372739
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Antigen
Synonyms cb358, wu:fc55h08, UCHL1, UCH-L1, park5, pgp9.5, uch-l1, MGC132191, uchl1, AW822034, C88048, PGP 9.5, PGP9.5, R75593, UCHL-1, gad, PARK5, PGP95, Uch-L1
Reactivity
Cow (Bovine), Human, Mammalian, Mouse (Murine), Rat (Rattus)
(226), (75), (71), (24), (21), (14), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host
Chicken
(140), (84), (14), (11), (7), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(201), (102), (66), (64), (30), (19), (16), (10), (4), (3), (2), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN372739
335.50 $
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Immunogen Recombinant full length human UCHL1 purified from E. coli.
Specificity Specific for the the ~24kDa UCHL1 protein.
Purification Ig Fraction
Alternative Name UCHL1 / PGP9.5
Background Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.
Alternate names: Neuron cytoplasmic protein 9.5, PGP 9.5, UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1
Gene ID 7345
NCBI Accession NP_004172
UniProt P09936
Research Area Tags/Labels, Cell/Tissue Markers
Application Notes Western blotting: 1/2,500. Immunofluorescence: 1/500. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody
anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody (2) anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody (Image 2)
Background publications Wilkinson, Lee, Deshpande et al.: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, 1989 (PubMed).

Doran, Jackson, Kynoch et al.: "Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis." in: Journal of neurochemistry, Vol. 40, Issue 6, pp. 1542-7, 1983 (PubMed).

Kurihara, Kikuchi, Wada et al.: "Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia." in: Human molecular genetics, Vol. 10, Issue 18, pp. 1963-70, 2001 (PubMed).

Liu, Fallon, Lashuel et al.: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, 2002 (PubMed).

Validation Images
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