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UCHL1 antibody (N-Term)

The Rabbit Polyclonal anti-UCHL1 antibody is suitable to detect UCHL1 in samples from Human and Mouse. It has been validated for WB, IF, FACS and IHC (p).

All lanes : Anti-UCHL1 Antibody (V31) at 1:1000 dilution Lane 1: D whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. (UCHL1 antibody (N-Term))

Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (ABIN388868 and ABIN2839164) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (UCHL1 antibody (N-Term))

IHC (p)

Confocal immunofluorescent analysis of UCHL1 Antibody (N-term) (ABIN388868 and ABIN2839164) with NCI- cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (UCHL1 antibody (N-Term))

Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 (ABIN388868 and ABIN2839164). (UCHL1 antibody (N-Term))

Western blot analysis of UCHL1-V31 (ABIN388868 and ABIN2839164) in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35 μg/lane). (UCHL1 antibody (N-Term))

UCHL1 Antibody (N-term) (ABIN388868 and ABIN2839164) flow cytometric analysis of NCI- cells (right histogram) compared to a negative control cell (left histogram). (UCHL1 antibody (N-Term))

FACS

6 images

Catalog No. ABIN388868

$451.00

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Quick Overview for UCHL1 antibody (N-Term) (ABIN388868)

Target

See all UCHL1 Antibodies

UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity

Human, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This UCHL1 antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB04183

Discover our top product UCHL1 Primary Antibody

Binding Specificity
52

15

13

13

9

7

5

4

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 16-46, N-Term

Predicted Reactivity

B, Pig

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human UCHL1.

Isotype

Ig Fraction
anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 187-216), (C-Term) antibody
UCHL1 Reactivity: Human, Rat, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB7379 unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 2-223) antibody
UCHL1 Reactivity: Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 2-223) antibody
UCHL1 Reactivity: Mouse WB, IHC, FACS, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 2-223) antibody
UCHL1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody
UCHL1 Reactivity: Human, Rat, Mouse, Dog, Hamster WB, IHC, IF, FACS Host: Mouse Monoclonal rUCHL1-775 unconjugated Recombinant Antibody

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 59-223) antibody
UCHL1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (C-Term) antibody
UCHL1 Reactivity: Human, Rat, Mouse WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 1-223) antibody
UCHL1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1B8-4D2 unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody
UCHL1 Reactivity: Human, Rat, Mouse, Cow, Dog, Hamster WB, IHC, IF, FACS Host: Mouse Monoclonal 31A3 unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 50-100) antibody
UCHL1 Reactivity: Human, Mouse WB, IHC, FACS, ICC Host: Rabbit Monoclonal BLR216K unconjugated Recombinant Antibody

Application Notes

IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name

PGP9.5 (UCHL1/PARK5)

Background

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).

Molecular Weight

24824

Gene ID

7345

NCBI Accession

NP_004172

UniProt

P09936

Pathways

Feeding Behaviour