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Details for Product No. ABIN374079

Neutrophil Cytosol Factor 1 (NCF1) (C-Term) antibody

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Synonyms NCF1A, NOXO2, SH3PXD1A, p47phox, Ncf-1, p47
»Alternatives C-Term
»Alternatives Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN374079
Quantity 0.1 mg
346.50 $   Plus shipping costs $45.00
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Immunogen Peptide from the C Terminus of the protein according to NP_000256.3. Genename: NCF1
Specificity This antibody detects NCF-1 at C-term. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Rabbit, Pig, Cow.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name NCF1
Background NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).
Alternate names: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K Neutrophil NADPH oxidase factor 1, NOXO2, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2 SH3 and PX domain-containing protein 1A p47-phox, SH3PXD1A
Gene ID 653361
NCBI Accession NP_000256.3
UniProt P14598
Research Area Cancer, Metabolism
Application Notes Peptide ELISA: Limit dilution 1: 20000. Western blot: 0.2-2 µg/ml. Approx 47kDa band seen in U937 cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).

Alternatives for antigen "Neutrophil Cytosol Factor 1 (NCF1)", type "Antibodies"
Hosts (126), (15)
Reactivities (130), (72), (63), (25), (18), (14), (4), (2), (2), (1), (1), (1)
Applications (96), (90), (63), (30), (23), (17), (4), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (31), (18), (8), (5), (5), (5), (4), (2), (2), (1), (1), (1)