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NCF1 antibody (Neutrophil Cytosol Factor 1) (C-Term)

Details for Product anti-NCF1 Antibody No. ABIN374079, Supplier: Log in to see
Antigen
  • NCF1A
  • NOXO2
  • SH3PXD1A
  • p47phox
  • Ncf-1
  • p47
Alternatives
anti-Human NCF1 antibody for Western Blotting
Epitope
C-Term
54
22
15
15
13
10
9
5
5
5
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
168
66
64
12
9
7
6
6
5
4
2
1
Host
Goat
155
26
2
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
5
5
5
3
3
3
3
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
140
88
84
24
23
21
10
4
4
2
1
Supplier
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Immunogen Peptide from the C Terminus of the protein according to NP_000256.3. Genename: NCF1
Sequence C-SESTKRKLAS AV
Specificity This antibody detects NCF-1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Rabbit, Pig, Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name NCF1 (NCF1 Antibody Abstract)
Background NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NOXO2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, SH3PXD1A, p47-phox
Gene ID 653361
NCBI Accession NP_000256
UniProt P14598
Research Area Cancer, Metabolism
Pathways PI3K-Akt Signaling
Application Notes Peptide ELISA: Limit dilution 1: 20000. Western blot: 0.2-2 μg/mL. Approx 47 kDa band seen in U937 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (C-Term) (ABIN374079) anti-Neutrophil Cytosol Factor 1 (NCF1) (C-Term) antibody
Background publications Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).