NCF1 antibody (pSer359)

Catalog No. ABIN1531723

This Rabbit Polyclonal antibody specifically detects NCF1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for NCF1 antibody (pSer359) (ABIN1531723)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NCF1 Antibody

Binding Specificity: AA 331-380, pSer359

Purpose: p47-phox (phospho Ser359) Antibody

Specificity: Phospho-p47-phox (S359) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S359.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359. AA range:331-380

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-NCF1 antibody (ABIN1531723)

Kim, Cho, Mun, Kim, Kim, Kim, Son, Jeon, Kim, Jeong, Jang, Kim, Yang: "Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis." in: Biomedicines, Vol. 9, Issue 5, (2021) (PubMed).

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: p47 Phox

Background:

Synonyms: NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3

Background: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],

Gene Name: NCF1

Protein Name: Neutrophil cytosol factor 1

Molecular Weight: 44 kDa

Gene ID: 653361

OMIM: 233700

UniProt: P14598

Pathways: PI3K-Akt Signaling