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Numb Homolog (NUMB) (C-Term) antibody

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Synonyms Nb, C14orf41, S171, c14_5527
(34), (32), (13), (13), (9), (8), (7), (6), (6), (6), (6), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
(141), (63), (25), (6), (5), (4), (2), (2), (2), (2), (1), (1)
(88), (52), (17), (3)
(5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(133), (42), (25), (22), (19), (12), (12), (12), (10), (6), (4), (3), (1)
Pubmed 8 references available
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Quantity 0.1 mg
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide from the C-Terminus of Human NUMB (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1) Genename: NUMB
Specificity This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity chromatography using the immunizing peptide
Alternative Name NUMB (NUMB Antibody Abstract)
Background Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelatorSynonyms: Protein S171, Protein numb homolog, h-Numb
Gene ID 8650
NCBI Accession NP_001005743
UniProt P49757
Research Area Stem Cells
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.03-0.1 μg/mL. Approx 80 kDa band observed in Mouse Brain lysates(calculated MW of 70.0 kDa according to NP_001005743.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Image no. 1 for anti-Numb Homolog (NUMB) (C-Term) antibody (ABIN374158) anti-Numb Homolog (NUMB) (C-Term) antibody
Background publications Sczaniecka, Gladstone, Pettersson et al.: "MDM2 protein-mediated ubiquitination of numb protein: identification of a second physiological substrate of MDM2 that employs a dual-site docking mechanism." in: The Journal of biological chemistry, Vol. 287, Issue 17, pp. 14052-68, 2012 (PubMed).

Euskirchen, Skaftnesmo, Huszthy et al.: "NUMB does not impair growth and differentiation status of experimental gliomas." in: Experimental cell research, Vol. 317, Issue 20, pp. 2864-73, 2011 (PubMed).

Ito, Kwon, Zimdahl et al.: "Regulation of myeloid leukaemia by the cell-fate determinant Musashi." in: Nature, Vol. 466, Issue 7307, pp. 765-8, 2010 (PubMed).

Kharas, Lengner, Al-Shahrour et al.: "Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia." in: Nature medicine, Vol. 16, Issue 8, pp. 903-8, 2010 (PubMed).

Ma, Zhou, Shi et al.: "NUMBL interacts with TAB2 and inhibits TNFalpha and IL-1beta-induced NF-kappaB activation." in: Cellular signalling, Vol. 20, Issue 6, pp. 1044-51, 2008 (PubMed).

Corallini, Fera, Grisanti et al.: "Expression of the adaptor protein m-Numb in mouse male germ cells." in: Reproduction (Cambridge, England), Vol. 132, Issue 6, pp. 887-97, 2006 (PubMed).

Di Marcotullio, Ferretti, Greco et al.: "Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination." in: Nature cell biology, Vol. 8, Issue 12, pp. 1415-23, 2006 (PubMed).

Holowacz, Zeng, Lassar: "Asymmetric localization of numb in the chick somite and the influence of myogenic signals." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 3, pp. 633-45, 2006 (PubMed).

Catalog No. ABIN374158
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