NUMB antibody (pSer276)

Catalog No. ABIN744428

Product Details anti-NUMB Antibody

Target: NUMB (Numb Homolog (NUMB))

Binding Specificity: pSer276

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is homologous to that of Ser276 in Mouse and Ser275 in Rat.

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Numb around the phosphorylation site of Ser276

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NUMB antibody (ABIN744428)

Mah, Soloff, Hedrick, Mariani: "Atypical PKC-iota Controls Stem Cell Expansion via Regulation of the Notch Pathway." in: Stem cell reports, Vol. 5, Issue 5, pp. 866-80, (2015) (PubMed).

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: Numb (NUMB Products)

Synonyms: C14orf41 antibody, S171 antibody, c14_5527 antibody, Nb antibody, NUMB, endocytic adaptor protein antibody, numb homolog (Drosophila) antibody, NUMB antibody, Numb antibody, numb antibody

Background:

Synonyms: S171, C14orf41, c14_5527, Protein numb homolog, h-Numb, Protein S171, NUMB

Background: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization