Alstrom Syndrome 1 (ALMS1) (C-Term) antibody

Details for Product No. ABIN374311
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Antigen
Synonyms ALMS1, ALSS, bbb
Epitope
C-Term
(7), (6), (2), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(20), (2), (2), (1), (1), (1), (1), (1)
Host
Goat
(16), (6)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(15), (14), (10), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374311
357.50 $
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Immunogen Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1
Sequence C-RVTNQLLGRK VPWD
Specificity This antibody detects ALMS1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name ALMS1
Background ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328
Gene ID 7840
NCBI Accession NP_055935
UniProt Q8TCU4
Research Area Immunology
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
anti-Alstrom Syndrome 1 (ALMS1) (C-Term) antibody anti-Alstrom Syndrome 1 (ALMS1) (C-Term) antibody
Background publications Collin, Marshall, Ikeda et al.: "Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alström syndrome." in: Nature genetics, Vol. 31, Issue 1, pp. 74-8, 2002 (PubMed).

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