SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody

Details for Product No. ABIN387798
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Antigen
Synonyms Qscn6, Qsox, Sox-2, rSOx, ANOP3, MCOPS3, RGD1565646, SRY-box2, Xsox-2, anop3, mcops3, sox-2, lcc, ysb, cb236, wu:fb83g04, wu:fc14d07, zgc:65860, zgc:77389
Reactivity
Human
(195), (65), (44), (20), (20), (20), (16), (12), (5), (4), (4), (3), (2), (2), (1), (1), (1)
Host
Mouse
(160), (41), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(11), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(143), (74), (39), (33), (31), (28), (26), (23), (10), (10), (8), (3), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN387798
291.50 $
Plus shipping costs $45.00

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Immunogen SOX2 recombinant protein is used to produce this monoclonal antibody.
Clone 57CT23-3-4
Isotype IgG1
Characteristics MW: 34310 Da predicted from amino acid sequence.
Theoretical pI=9.74 Da
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name SOX2
Background This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Synonyms: Transcription factor SOX-2
Molecular Weight 34310 Da
Gene ID 6657
UniProt P48431
Research Area Embryogenesis, Embryonic stem cells, Reproduction, Transcription Factors, Neural Stem Cell marker, Stem Cells, Signaling
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:200-2000, IHC = 1:50-100, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (LEFT)Western blot analysis of anti-SOX2 Monoclonal Antibody (ABIN387798) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab. (RIGHT)Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (ABIN387798). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies) (LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Monoclonal Antibody (ABIN387798), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (MIDDLE)Immunofluorescence analysis of SOX2 Monoclonal antibody (ABIN387798) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-mouse lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. (RIGHT)Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (2) anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (Image 2)
Background publications Reis, Tyler, Schneider et al.: "Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes." in: Molecular vision, Vol. 16, pp. 768-73, 2010 (PubMed).

Gonzalez-Rodriguez, Pelcastre, Tovilla-Canales et al.: "Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases." in: The British journal of ophthalmology, Vol. 94, Issue 8, pp. 1100-4, 2010 (PubMed).

Ge, Zhou, Chen et al.: "Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells." in: Biochemical and biophysical research communications, Vol. 397, Issue 4, pp. 711-7, 2010 (PubMed).

Sholl, Barletta, Yeap et al.: "Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma." in: The American journal of surgical pathology, Vol. 34, Issue 8, pp. 1193-8, 2010 (PubMed).

Zhang, Efendic, Brismar et al.: "Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus." in: BMC medical genetics, Vol. 11, pp. 116, 2010 (PubMed).

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