DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody

Details for Product No. ABIN387881
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Antigen
Synonyms DNMT3A2, M.HsaIIIA, DNMT3A
Epitope
AA 457-486
(21), (8), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(106), (30), (23), (13), (1), (1)
Host
Rabbit
(97), (10), (3), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(99), (53), (25), (13), (10), (9), (7), (4), (3), (2)
Pubmed 2 references available
Catalog no. ABIN387881
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 AA from human Dnmt3a.
Clone RB01852
Isotype Ig
Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465~494 amino acids from the central region of human Dnmt3a.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Dnmt3a
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Synonyms: DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA
Molecular Weight 101558 DA
Gene ID 1788
UniProt Q9Y6K1
Research Area Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody Western blot analysis of anti-Dnmt3a Pab (ABIN387881) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody (2) Lysates from thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals. FR, animals subjected to fractionated exposure. AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown. each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01.3(10):553-561)
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Robertson, Uzvolgyi, Liang et al.: "The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors." in: Nucleic acids research, Vol. 27, Issue 11, pp. 2291-8, 1999 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Hosts (97), (10), (3), (2)
Reactivities (106), (30), (23), (13), (1), (1)
Applications (99), (53), (25), (13), (10), (9), (7), (4), (3), (2)
Conjugates (7), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (21), (8), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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