Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term), (AA 415-445) antibody

Details for Product No. ABIN387909
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Antigen
Synonyms Ube1c, AI256736, AI848246, AW546539, A830034N06Rik, ube1c, zgc:55528, wu:fb75e04, wu:fc37b11, UBE1C, hUBA3
Epitope
C-Term, AA 415-445
(3), (2), (1)
Reactivity
Human, Mouse (Murine)
(11), (3), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(11)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(10), (4), (4), (3)
Pubmed 5 references available
Catalog no. ABIN387909
Quantity 400 µL
Price
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Immunogen This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 AA from the C-terminal region of human UBE1C.
Clone RB2803
Isotype Ig
Specificity This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423~452 amino acids from the C-terminal region of human UBE1C.
Predicted Reactivity Rat (Rattus),Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name UBE1C
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Synonyms: NEDD8-activating enzyme E1 catalytic subunit, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, UBA3
Molecular Weight 51719 DA
Gene ID 9039
UniProt Q8TBC4
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term), (AA 415-445) antibody The anti-UBE1 C-term Pab (ABIN387909) is used in Western blot to detect UBE1 in mouse brain tissue lysate.
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term), (AA 415-445) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term), (AA 415-445) antibody (3) UBE1C Antibody (S430) (ABIN387909) western blot analysis in HL-60 cell line lysates (35 µg/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
Background publications Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).

Gubin, Njoroge, Bouffard et al.: "Gene expression in proliferating human erythroid cells." in: Genomics, Vol. 59, Issue 2, pp. 168-77, 1999 (PubMed).

Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).

Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).

Hosts (11)
Reactivities (11), (3), (1), (1), (1), (1), (1), (1)
Applications (10), (4), (4), (3)
Epitopes (3), (2), (1)
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