UBA3 antibody (Internal Region)

Catalog No. ABIN6265835

Product Details anti-UBA3 Antibody

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: UBA3 Antibody detects endogenous levels of total UBA3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UBA3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UBA3

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Alternative Name: UBA3 (UBA3 Products)

Synonyms: UBE1C antibody, hUBA3 antibody, Ube1c antibody, ube1c antibody, wu:fb75e04 antibody, wu:fc37b11 antibody, zgc:55528 antibody, A830034N06Rik antibody, AI256736 antibody, AI848246 antibody, AW546539 antibody, ubiquitin like modifier activating enzyme 3 antibody, ubiquitin-like modifier activating enzyme 3 antibody, ubiquitin like modifier activating enzyme 3 S homeolog antibody, UBA3 antibody, Uba3 antibody, uba3 antibody, uba3.S antibody

Background:

Description: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

Gene: UBA3

Molecular Weight: 49 kDa

Gene ID: 9039

UniProt: Q8TBC4