SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody

Details for Product No. ABIN387913
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Synonyms ARX, SAE2, Sae2, Uble1b, ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, sae2, sae2-B, uba2-a, uba2-b, uble1b-B
C-Term, AA 601-631
(9), (3), (2), (1), (1), (1), (1)
(41), (7), (4), (1), (1)
(34), (5), (3)
Clonality (Clone)
Polyclonal ()
Western Blotting (WB), Immunohistochemistry (IHC)
(36), (25), (15), (11), (4), (2), (1)
Pubmed 3 references available
Catalog no. ABIN387913
Quantity 400 µL
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Immunogen This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 601-631 AA from the C-terminal region of human SAE2 (UBA2).
Clone RB2822
Isotype Ig
Specificity This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 609~638 amino acids from the C-terminal region of human UBA2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SAE2 (UBA2)
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
Molecular Weight 71223 DA
Gene ID 10054
UniProt Q9UBT2
Research Area Protein Modifications, Cancer, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody The anti-UBA2 C-term E616 Antibody (ABIN387913) is used in Western blot to detect UBA2 in Jurkat lysate.
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Hosts (34), (5), (3)
Reactivities (41), (7), (4), (1), (1)
Applications (36), (25), (15), (11), (4), (2), (1)
Epitopes (9), (3), (2), (1), (1), (1), (1)
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