SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody

Details for Product No. ABIN387913
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Antigen
Synonyms
ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, ARX, SAE2, AA986091, Arx, Sae2, UBA1, Ubl1a2, Uble1b, sae2, sae2-B, uba2-a, uba2-b, uble ... show more
ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, ARX, SAE2, AA986091, Arx, Sae2, UBA1, Ubl1a2, Uble1b, sae2, sae2-B, uba2-a, uba2-b, uble1b-B, sae2b, uble1b, wu:fi17g06, zgc:66354 show less
Epitope
C-Term, AA 601-631
(16), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(51), (6), (3), (1), (1)
Host
Rabbit
(43), (5), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(46), (32), (18), (17), (9), (2), (2), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN387913
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Immunogen This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 601-631 AA from the C-terminal region of human SAE2 (UBA2).
Clone RB2822
Isotype Ig
Specificity This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 609~638 amino acids from the C-terminal region of human UBA2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SAE2 (UBA2)
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
Molecular Weight 71223 DA
Gene ID 10054
UniProt Q9UBT2
Research Area Protein Modifications, Cancer, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody The anti-UBA2 C-term E616 Antibody (ABIN387913) is used in Western blot to detect UBA2 in Jurkat lysate.
anti-SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (AA 601-631) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Validation Images
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