Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30), (Thr11) antibody

Details for Product No. ABIN388774
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Antigen
Synonyms IPF1, PDX-1, ipf1, pdx-1, zgc:100874, XlHbox-8, xlhbox8, GSF, IUF1, IDX-1, MODY4, STF-1, IPF-1, Ipf1, Mody4, Idx1, Stf1, PDX1
Epitope
AA 1-30, Thr11
(18), (15), (15), (9), (8), (6), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(100), (39), (26), (14), (14), (6), (3), (1), (1), (1)
Host
Rabbit
(94), (17), (5), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
(75), (75), (31), (16), (15), (10), (6), (6), (3), (3), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN388774
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from human PDX1.
Clone RB15204
Isotype Ig
Specificity This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids surrounding T11 of human PDX1.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PDX1
Background PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Synonyms: Pyruvate dehydrogenase protein X component, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein
Molecular Weight 54122 Da predicted from amino acid sequence. Theoretical pI: 8.80 Da
Gene ID 8050
UniProt O00330
Research Area Stem Cells, Metabolism, Cell Structure
Application Notes IF = 1:100, WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30), (Thr11) antibody Western blot analysis of anti-PDX1 Antibody (T11) (ABIN388774) in NCI-H460 cell line lysates (35 µg/lane).PDX1-T11(arrow) was detected using the purified polyclonal antibody.
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30), (Thr11) antibody (2) Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11) (ABIN388774). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30), (Thr11) antibody (3) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Harris, Bowker-Kinley, Wu et al.: "Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 19746-51, 1997 (PubMed).

Aral, Benelli, Ait-Ghezala et al.: "Mutations in PDX1, the human lipoyl-containing component X of the pyruvate dehydrogenase-complex gene on chromosome 11p1, in congenital lactic acidosis." in: American journal of human genetics, Vol. 61, Issue 6, pp. 1318-26, 1998 (PubMed).

Ling, McEachern, Seyda et al.: "Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency." in: Human molecular genetics, Vol. 7, Issue 3, pp. 501-5, 1998 (PubMed).

Hosts (94), (17), (5), (2)
Reactivities (100), (39), (26), (14), (14), (6), (3), (1), (1), (1)
Applications (75), (75), (31), (16), (15), (10), (6), (6), (3), (3), (2), (2), (1), (1)
Conjugates (6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (15), (15), (9), (8), (6), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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