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MIB1 antibody (Mindbomb Homolog 1 (Drosophila)) (AA 13-42)

Details for Product anti-MIB1 Antibody No. ABIN388956, Supplier: Log in to see
AA 13-42, N-Term
Clonality (Clone)
Polyclonal ()
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 AA from the N-terminal region of human Mib1/Mindbomb.
Clone RB4448
Isotype Ig
Specificity This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human MIB.
Predicted Reactivity Mouse (Murine),Xenopus laevis,Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Mib1/Mindbomb
Background MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Synonyms: E3 ubiquitin-protein ligase MIB1, Mind bomb homolog 1, DAPK-interacting protein 1, DIP-1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2
Molecular Weight 110135 DA
Gene ID 57534
UniProt Q86YT6
Research Area Ubiquitin-related antibodies, Translation Factors, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Neurology
Application Notes WB = 1:1000, IF = 1:20-100, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
 image for anti-MIB1 antibody (Mindbomb Homolog 1 (Drosophila)) (AA 13-42) (ABIN388956) The Mib Pab (ABIN388956) is used at 1:300 to detect DYKDDDDK-tagged Mib in 293T cell ...
Immunofluorescence (IF) image for anti-MIB1 antibody (Mindbomb Homolog 1 (Drosophila)) (AA 13-42) (ABIN388956) Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (ABIN38...
Immunohistochemistry (IHC) image for anti-MIB1 antibody (Mindbomb Homolog 1 (Drosophila)) (AA 13-42) (ABIN388956) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Itoh, Kim, Palardy, Oda, Jiang, Maust, Yeo, Lorick, Wright, Ariza-McNaughton, Weissman, Lewis, Chandrasekharappa, Chitnis: "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." in: Developmental cell, Vol. 4, Issue 1, pp. 67-82, 2003 (PubMed).

Jin, Blue, Dixon, Shao, Gallagher: "A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK." in: The Journal of biological chemistry, Vol. 277, Issue 49, pp. 46980-6, 2002 (PubMed).