Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term), (AA 13-42) antibody

Details for Product No. ABIN388956
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Antigen
Epitope
N-Term, AA 13-42
(1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN388956
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Immunogen This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 AA from the N-terminal region of human Mib1/Mindbomb.
Clone RB4448
Isotype Ig
Specificity This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human MIB.
Predicted Reactivity Mouse (Murine),Xenopus laevis,Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Mib1/Mindbomb
Background MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Synonyms: E3 ubiquitin-protein ligase MIB1, Mind bomb homolog 1, DAPK-interacting protein 1, DIP-1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2
Molecular Weight 110135 DA
Gene ID 57534
UniProt Q86YT6
Research Area Ubiquitin-related antibodies, Translation Factors, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Neurology
Application Notes WB = 1:1000, IF = 1:20-100, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term), (AA 13-42) antibody The Mib Pab (ABIN388956) is used at 1:300 to detect DYKDDDDK-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.
anti-Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term), (AA 13-42) antibody (2) Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (ABIN388956) at a dilution of 1:20.
anti-Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term), (AA 13-42) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody staining.
Background publications Jin, Blue, Dixon et al.: "A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK." in: The Journal of biological chemistry, Vol. 277, Issue 49, pp. 46980-6, 2002 (PubMed).

Itoh, Kim, Palardy et al.: "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." in: Developmental cell, Vol. 4, Issue 1, pp. 67-82, 2003 (PubMed).

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