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Details for Product No. ABIN388956

Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term), (AA 13-42) antibody

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Antigen
Epitope
N-Term, AA 13-42
Reactivity
Human
Host
Rabbit
Clonality (Clone) Polyclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN388956
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 AA from the N-terminal region of human Mib1/Mindbomb.
Clone RB4448
Isotype Ig
Specificity This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human MIB.
Predicted Reactivity Mouse (Murine),Xenopus laevis,Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Mib1/Mindbomb
Background MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Synonyms: E3 ubiquitin-protein ligase MIB1, Mind bomb homolog 1, DAPK-interacting protein 1, DIP-1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2
Molecular Weight 110135 DA
Gene ID 57534
UniProt Q86YT6
Research Area Ubiquitin-related antibodies, Translation Factors, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Neurology
Application Notes Recommend dilutions: WB : 1:1000,IF : 1:20-100,IHC : 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Jin, Blue, Dixon et al.: "A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK." in: The Journal of biological chemistry, Vol. 277, Issue 49, pp. 46980-6, 2002 (PubMed).

Itoh, Kim, Palardy et al.: "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." in: Developmental cell, Vol. 4, Issue 1, pp. 67-82, 2003 (PubMed).

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