BMI (AA 257-286), (C-Term) antibody

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AA 257-286, C-Term
Clonality (Clone)
Polyclonal ()
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 AA from the C-terminal region of human BMI1.
Clone RB02926
Isotype Ig
Specificity This BMI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the C-terminal region of human BMI.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.
Synonyms: Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51
Molecular Weight 36949 DA
Gene ID 648
UniProt P35226
Research Area Ubiquitin-related antibodies, Stem Cells, Protein Modifications, Cancer, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BMI (AA 257-286), (C-Term) antibody (ABIN389084) Western blot analysis of hBMI1-C271 antibody(ABIN389084) in 293 cell line lysates (35...
Immunohistochemistry (IHC) image for anti-BMI (AA 257-286), (C-Term) antibody (ABIN389084) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI Antibody ...
Western Blotting (WB) image for anti-BMI (AA 257-286), (C-Term) antibody (ABIN389084) BMI1 Antibody (C271) (ABIN389084) western blot analysis in K562 cell line lysates (35...
Product cited in: Wang, Venugopal, Manoranjan, McFarlane, OFarrell, Nolte, Gunnarsson, Hollenberg, Kwiecien, Northcott, Taylor, Hawkins, Singh: "Sonic hedgehog regulates Bmi1 in human medulloblastoma brain tumor-initiating cells." in: Oncogene, Vol. 31, Issue 2, pp. 187-99, 2012 (PubMed).

Lewis, Segditsas, Deheragoda, Pollard, Jeffery, Nye, Lockstone, Davis, Clark, Stamp, Poulsom, Wright, Tomlinson: "Severe polyposis in Apc(1322T) mice is associated with submaximal Wnt signalling and increased expression of the stem cell marker Lgr5." in: Gut, Vol. 59, Issue 12, pp. 1680-6, 2010 (PubMed).

Sangiorgi, Capecchi: "Bmi1 lineage tracing identifies a self-renewing pancreatic acinar cell subpopulation capable of maintaining pancreatic organ homeostasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 17, pp. 7101-6, 2009 (PubMed).

Background publications Xia, Anderson, Diaz, Zeleznik-Le: "MLL repression domain interacts with histone deacetylases, the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 14, pp. 8342-7, 2003 (PubMed).

Bárdos, Saurin, Tissot, Duprez, Freemont: "HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28785-92, 2000 (PubMed).

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