DNA-PK (pThr2609) antibody

Details for Product No. ABIN389868
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Antigen
Epitope
pThr2609
(6), (6), (3), (3), (3), (2), (1)
Reactivity
Human
(44), (24), (2), (2)
Host
Rabbit
(43), (1)
Clonality (Clone)
Polyclonal ()
Application
Dot Blot (DB)
(36), (28), (27), (13), (7), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN389868
291.50 $
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Immunogen This DNA-PK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.
Clone RB8113
Isotype Ig
Specificity This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.
Synonyms: DNA-dependent protein kinase catalytic subunit, DNA-PKcs, DNPK1
Molecular Weight 469079 DA
Gene ID 5591
UniProt P78527
Research Area Cell Signaling, Cell Structure, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Metabolism
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DNA-PK (pThr2609) antibody Dot blot analysis of anti-DNA-PK-pT2609 Pab (ABIN389868) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
Background publications Goudelock, Jiang, Pereira et al.: "Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29940-7, 2003 (PubMed).

Calsou, Delteil, Frit et al.: "Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment." in: Journal of molecular biology, Vol. 326, Issue 1, pp. 93-103, 2003 (PubMed).

Karpova, Trost, Murray et al.: "Interferon regulatory factor-3 is an in vivo target of DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2818-23, 2002 (PubMed).

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