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DNA-PK (pThr2609) antibody

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Antigen
Epitope
»Alternatives pThr2609
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives Dot Blot (DB), ELISA
Pubmed 3 references available
Catalog no. ABIN389868
Quantity 400 µL
Price
280.50 $   Plus shipping costs $45.00
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anti-DNA-PK (pThr2609) antibody

Product details

Target Details Application Details Handling Images References Alternatives back to top
Immunogen This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.
Specificity This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.
Purification This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Target Details

Application Details Handling Images References Alternatives back to top
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.
Synonyms: DNA-dependent protein kinase catalytic subunit, DNA-PKcs, DNPK1
Molecular Weight 469079 DA
Gene ID 5591
UniProt P78527
Research Area Cell Signaling, Cell Structure, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Metabolism

Application Details

Target Details Handling Images References Alternatives back to top
Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500
Comment

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.
Restrictions For Research Use only

Handling

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Concentration 0.25 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 2-8 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References

Target Details Application Details Handling Images Alternatives back to top
Product cited in: Karpova, Trost, Murray et al.: "Interferon regulatory factor-3 is an in vivo target of DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2818-23, 2002 (PubMed).

Calsou, Delteil, Frit et al.: "Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment." in: Journal of molecular biology, Vol. 326, Issue 1, pp. 93-103, 2003 (PubMed).

Goudelock, Jiang, Pereira et al.: "Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29940-7, 2003 (PubMed).

Alternatives

Target Details Application Details Handling Images References back to top
Alternatives for antigen "DNA-PK", type "Antibodies"
Hosts (43)
Reactivities (43), (18), (2), (2)
Applications (37), (27), (20), (8), (1), (1)
Epitopes (4), (3), (3), (3), (3), (1), (1)
Beacon
Guglhupf
Guglhupf Guglhupf Guglhupf