DNA-PK (pThr2609) antibody

Antigen: DNA-PK

Binding Site: pThr2609

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389868

Additional Information

Gene ID: 5591

UniProt: P78527

Immunogen: This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.

Isotype: Ig

Description: Other names: DNA-dependent protein kinase catalytic subunit, DNA-PKcs, DNPK1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.

Molecular Weight: 469079 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

Dot blot analysis of anti-DNA-PK-pT2609 Pab (ABIN389868) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Karpova, Trost, Murray et al.: "Interferon regulatory factor-3 is an in vivo target of DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2818-23, 2002 (PubMed).

Calsou, Delteil, Frit et al.: "Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment." in: Journal of molecular biology, Vol. 326, Issue 1, pp. 93-103, 2003 (PubMed).

Goudelock, Jiang, Pereira et al.: "Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29940-7, 2003 (PubMed).