Neuregulin 1 (NRG1) (AA 198-229), (Center) antibody

Details for Product No. ABIN390160
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, NDF, SMDF, Hgl, GGFII, HRGalpha, 6030402G23Rik, D230005F13Rik, pro-neuregulin-1, HPRG, NRG-1, heregulin, nrg1-A, NRG1, wu:fm70e01, DKFZp459A1838
Epitope
AA 198-229, Center
(7), (6), (6), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(83), (46), (37), (12), (12), (12), (12), (1), (1)
Host
Rabbit
(85), (20)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(88), (39), (37), (24), (13), (11), (10), (10), (6), (4), (3), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN390160
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This NRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-229 AA from the Central region of human NRG1.
Clone RB01275-01276
Isotype Ig
Specificity This NRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NRG1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NRG1
Background Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains, and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
Molecular Weight 70260 DA
Gene ID 3084
UniProt Q02297
Research Area Stem Cells, Cell Structure, Cell Cycle, Neurology
Application Notes WB = 1:1000, FACS = 1:10-50, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.79 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Neuregulin 1 (NRG1) (AA 198-229), (Center) antibody Western blot analysis of anti-NRG1 Antibody (Center) (ABIN390160) in SK-BR-3 cell line lysates (35 µg/lane). NRG1 (arrow) was detected using the purified polyclonal antibody.
anti-Neuregulin 1 (NRG1) (AA 198-229), (Center) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Neuregulin 1 (NRG1) (AA 198-229), (Center) antibody (3) Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Atlas, Cardillo, Mehmi et al.: "Heregulin is sufficient for the promotion of tumorigenicity and metastasis of breast cancer cells in vivo." in: Molecular cancer research : MCR, Vol. 1, Issue 3, pp. 165-75, 2003 (PubMed).

Ritch, Carroll, Sontheimer: "Neuregulin-1 enhances motility and migration of human astrocytic glioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20971-8, 2003 (PubMed).

Vermeer, Einwalter, Moninger et al.: "Segregation of receptor and ligand regulates activation of epithelial growth factor receptor." in: Nature, Vol. 422, Issue 6929, pp. 322-6, 2003 (PubMed).

Hosts (85), (20)
Reactivities (83), (46), (37), (12), (12), (12), (12), (1), (1)
Applications (88), (39), (37), (24), (13), (11), (10), (10), (6), (4), (3), (2), (2), (1), (1)
Conjugates (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (6), (6), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top