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Sirtuin 3 (SIRT3) (C-Term), (AA 250-279) antibody

Details for Product No. ABIN390178
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Antigen
Synonyms zgc:158774, sirtuin, SIR2L3, 2310003L23Rik, AI848213, Sir2l3
Epitope
C-Term, AA 250-279
(33), (7), (7), (7), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(85), (40), (26), (14), (12), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(83), (8), (7), (6), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(91), (62), (24), (15), (10), (10), (7), (3), (3), (1)
Pubmed 2 references available
Quantity 400 µL
Options
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Catalog No. ABIN390178
238.50 $
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Immunogen This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 AA from the C-terminal region of human SIRT3.
Clone RB01967
Isotype Ig
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SIRT3
Background Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

Cellular Location
Mitochondrion matrix.

Tissue Location
Widely expressed.

Synonyms: SIR2-like protein 3, mSIR2L3, NAD-dependent deacetylase sirtuin-3,Sir2l3, Sirt3
Molecular Weight 43573 Da
Gene ID 23410
UniProt Q9NTG7
Research Area Cancer, Proteases, Cell Structure
Application Notes Suggested dilutions:
WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Sirtuin 3 (SIRT3) (C-Term), (AA 250-279) antibody Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Sirtuin 3 (SIRT3) (C-Term), (AA 250-279) antibody (2) Western blot analysis of SIRT3 antibody (ABIN390178) in Y79 cell line lysates (35 µg/lane). SIRT3 (arrow) was detected using the purified polyclonal antibody.
anti-Sirtuin 3 (SIRT3) (C-Term), (AA 250-279) antibody (3) Sirt3 Antibody (G265) (ABIN390178) western blot analysis in mouse kidney tissue lysates (35 µg/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow).
General Pillai, Sundaresan, Kim et al.: "Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3133-44, 2010 (PubMed).

Kawamura, Uchijima, Horike et al.: "Sirt3 protects in vitro-fertilized mouse preimplantation embryos against oxidative stress-induced p53-mediated developmental arrest." in: The Journal of clinical investigation, 2010 (PubMed).

Validation Images
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