SIRT3 antibody (C-Term)

Catalog No. ABIN652184

There are 2+ publications for this product available. The Rabbit Polyclonal anti-SIRT3 antibody is suitable to detect SIRT3 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for SIRT3 antibody (C-Term) (ABIN652184)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB25945

Product Details anti-SIRT3 Antibody

Binding Specificity: AA 304-334, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:64000. WB: 1:2000. WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SIRT3 antibody (ABIN652184)

Chen, Liu, Li, Wang, Liu, Li, Zhang, Bu: "Mouse SIRT3 attenuates hypertrophy-related lipid accumulation in the heart through the deacetylation of LCAD." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118909, (2015) (PubMed).

Kawamura, Uchijima, Horike, Tonami, Nishiyama, Amano, Asano, Kurihara, Kurihara: "Sirt3 protects in vitro-fertilized mouse preimplantation embryos against oxidative stress-induced p53-mediated developmental arrest." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2817-28, (2010) (PubMed).

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: Sirt3

Background: SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

Molecular Weight: 36615

Gene ID: 64384

NCBI Accession: NP_001120823, NP_001171275, NP_071878

UniProt: Q8R104