Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term), (AA 27-56) antibody

Details for Product No. ABIN390473
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Antigen
Epitope
N-Term, AA 27-56
(6), (4)
Reactivity
Human
(31), (18), (17), (11), (11), (11), (3), (1)
Host
Rabbit
(24), (6), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(20), (9), (9), (8), (5), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN390473
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Immunogen This AGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 AA from the N-terminal region of human AGO1.
Clone RB18965
Isotype Ig
Specificity This AGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34~64 amino acids from the N-terminal region of human AGO1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name AGO1
Background AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
Synonyms: Protein argonaute-1, Argonaute1, hAgo1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99, AGO1, EIF2C1
Molecular Weight 97214 DA
Gene ID 26523
UniProt Q9UL18
Research Area Transcription Factors, Signaling
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term), (AA 27-56) antibody Western blot analysis of AGO1 Antibody (N-term) (ABIN390473) in K562 cell line lysates (35 µg/lane). AGO1 (arrow) was detected using the purified polyclonal antibody.
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term), (AA 27-56) antibody (2) AGO1 Antibody (N-term) (ABIN390473) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
Background publications Horikawa, Wood, Yang et al.: "Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7956-62, 2008 (PubMed).

Weinmann, Höck, Ivacevic et al.: "Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs." in: Cell, Vol. 136, Issue 3, pp. 496-507, 2009 (PubMed).

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