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Myocyte Enhancer Factor 2A (MEF2A) (pThr319) antibody

Details for Product No. ABIN401543, Supplier: Login to see
Antigen
Alternatives
anti-Human Myocyte Enhancer Factor 2A antibody for Western Blotting
Epitope
pThr319
40
37
25
19
18
16
13
13
10
9
9
8
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
246
126
107
9
7
5
5
3
2
1
Host
Rabbit
212
36
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF)
152
116
79
74
54
24
20
7
6
3
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human MEF2A around the phosphorylation site of Thr319 (V-T-TP-P-S).
Specificity This antibody detects endogenous levels of MEF2A only when phosphorylated at Thr319.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MEF2A (MEF2A Antibody Abstract)
Background The myocyte enhancer factor-2 (MEF-2) family of transcription factors associated with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF-4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Gene ID 4205
NCBI Accession NP_001124398
UniProt Q02078
Research Area Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors, Neurogenesis
Pathways Neurotrophin Signaling Pathway
Application Notes Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Myocyte Enhancer Factor 2A (MEF2A) (pThr319) antibody (ABIN401543) anti-Myocyte Enhancer Factor 2A (MEF2A) (pThr319) antibody
Background publications Dang: "c-Myc target genes involved in cell growth, apoptosis, and metabolism." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 1-11, 1999 (PubMed).

Martin, Miano, Hustad et al.: "A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 1647-56, 1994 (PubMed).

Henson, Walker: "The structure of nucleohistone. Hydrodynamic behaviour at high ionic strength." in: European journal of biochemistry / FEBS, Vol. 22, Issue 1, pp. 1-4, 1971 (PubMed).

Yu, Breitbart, Smoot et al.: "Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors." in: Genes & development, Vol. 6, Issue 9, pp. 1783-98, 1992 (PubMed).