JNK1/2 (pThr183), (pTyr185) antibody

Details for Product No. ABIN401562
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Antigen
Epitope
pThr183, pTyr185
(3), (3), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(7), (5), (5)
Host
Rabbit
(5), (2)
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(7), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN401562
357.50 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human JNK1/JNK2 around the phosphorylation site of Thr183/Tyr185 (M-M-Tp-P-YP-V-V).
Specificity This antibody detects endogenous levels of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Background JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.
Alternate names: JNK-46, JNK-55, JNK1/JNK2, MAPK8, MAPK9, PRKM8, PRKM9, SAPK1, c-Jun N-terminal kinase 1, c-Jun N-terminal kinase 2
Gene ID 5599
NCBI Accession NP_002741
UniProt P45983
Application Notes Western blot: 1/500~1/1000. Immunofluorescence: 1/100~1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-JNK1/2 (pThr183), (pTyr185) antibody anti-JNK1/2 (pThr183), (pTyr185) antibody
anti-JNK1/2 (pThr183), (pTyr185) antibody (2) anti-JNK1/2 (pThr183), (pTyr185) antibody (Image 2)
Background publications Davis: "Signal transduction by the c-Jun N-terminal kinase." in: Biochemical Society symposium, Vol. 64, pp. 1-12, 1999 (PubMed).

Ichijo: "From receptors to stress-activated MAP kinases." in: Oncogene, Vol. 18, Issue 45, pp. 6087-93, 1999 (PubMed).

Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, 2001 (PubMed).

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