DLG4 antibody

Catalog No. ABIN453959

Product Details anti-DLG4 Antibody

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Rat, Human, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects an ~100 kDa protein corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots. An additional protein of > 100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat, Bovine, Human

Characteristics: Synonyms: DLGH4, PSD-95, Disks large homolog 4, SAP90, SAP-90, Postsynaptic density protein 95,Synapse-associated protein 90

Purification: Protein G chromatography

Immunogen: Recombinant rat PSD-95

Clone: 6G6

Isotype: IgG2a

Application Details

Application Notes: Western blot: 1: 250. Immunocytochemistry. Immunoflourescence. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, in 0.09 % azide in 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: DLG4 / PSD95 (DLG4 Products)

Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90 kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an Nterminal variable segment followed by three aminoterminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).Synonyms: DLGH4, Disks large homolog 4, PSD-95, Postsynaptic density protein 95, SAP-90, SAP90, Synapse-associated protein 90

Gene ID: 29495

UniProt: P31016

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity