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Interferon Induced with Helicase C Domain 1 (IFIH1) (Center) antibody

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Synonyms MDA5, Hlcd, IDDM19, MDA-5, RLR-2, 9130009C22Rik, Helicard, RGD1560496
(18), (18), (12), (7), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(109), (42), (4), (2), (1)
(73), (33), (22)
(3), (2), (2), (2), (2), (2)
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(87), (61), (43), (19), (11), (6), (5), (3), (2), (1), (1), (1)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen 16 amino acid peptide from near the center of Human MDA5.
Isotype IgG
Specificity This antibody detects Helicard at Center.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse
Purification Peptide Affinity Chromatography
Alternative Name Helicard (IFIH1 Antibody Abstract)
Background The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116
Gene ID 64135
NCBI Accession NP_071451
UniProt Q9BYX4
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Interferon Induced with Helicase C Domain 1 (IFIH1) (Center) antibody (ABIN500266) Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 Antibody  at 20 mg/ml
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interferon Induced with Helicase C Domain 1 (IFIH1) (Center) antibody (ABIN500266) Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/ml.
Western Blotting (WB) image for anti-Interferon Induced with Helicase C Domain 1 (IFIH1) (Center) antibody (ABIN500266) Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2...
Background publications Hiscott, Nguyen, Arguello et al.: "Manipulation of the nuclear factor-kappaB pathway and the innate immune response by viruses." in: Oncogene, Vol. 25, Issue 51, pp. 6844-67, 2006 (PubMed).

Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).

Akira, Uematsu, Takeuchi: "Pathogen recognition and innate immunity." in: Cell, Vol. 124, Issue 4, pp. 783-801, 2006 (PubMed).

Andrejeva, Childs, Young et al.: "The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17264-9, 2004 (PubMed).

Kang, Gopalkrishnan, Lin et al.: "Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene." in: Oncogene, Vol. 23, Issue 9, pp. 1789-800, 2004 (PubMed).

Benesh, Liyanage: "Surface-embedded Green-function method for general surfaces: Application to Al(111)." in: Physical review. B, Condensed matter, Vol. 49, Issue 24, pp. 17264-17272, 1999 (PubMed).

Catalog No. ABIN500266
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