Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN500266

Interferon Induced with Helicase C Domain 1 (IFIH1) (Center) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms Hlcd, IDDM19, MDA-5, MDA5, RLR-2, 9130009C22Rik, Helicard, RGD1560496
Epitope
»Alternatives Center
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN500266
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen 16 amino acid peptide from near the center of Human MDA5.
Isotype IgG
Specificity This antibody detects Helicard at Center. Species Reactivity: Tested: Human, Mouse
Purification Peptide Affinity Chromatography
Alternative Name Helicard
Background The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Alternate names: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down- regulated protein, RH116, RNA helicase-DEAD box protein 116
Gene ID 64135
NCBI Accession NP_071451
UniProt Q9BYX4
Application Notes ELISA. Western blot: 1 – 2 μg/ml. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Benesh, Liyanage: "Surface-embedded Green-function method for general surfaces: Application to Al(111)." in: Physical review. B, Condensed matter, Vol. 49, Issue 24, pp. 17264-17272, 1999 (PubMed).

Kang, Gopalkrishnan, Lin et al.: "Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene." in: Oncogene, Vol. 23, Issue 9, pp. 1789-800, 2004 (PubMed).

Andrejeva, Childs, Young et al.: "The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17264-9, 2004 (PubMed).

Akira, Uematsu, Takeuchi: "Pathogen recognition and innate immunity." in: Cell, Vol. 124, Issue 4, pp. 783-801, 2006 (PubMed).

Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).

Hiscott, Nguyen, Arguello et al.: "Manipulation of the nuclear factor-kappaB pathway and the innate immune response by viruses." in: Oncogene, Vol. 25, Issue 51, pp. 6844-67, 2006 (PubMed).

Alternatives for antigen "Interferon Induced with Helicase C Domain 1 (IFIH1)", type "Antibodies"
Hosts (47), (12), (4)
Reactivities (54), (35), (4), (1), (1), (1), (1), (1)
Applications (51), (34), (20), (16), (3), (3), (1), (1), (1)
Epitopes (11), (6), (5), (4), (3), (1), (1), (1), (1)
Beacon