Lysocardiolipin Acyltransferase 1 (LCLAT1) (Middle Region) antibody

Details for Product No. ABIN502511
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Antigen
Synonyms lclat1, zgc:77380, wu:fj17g04, LYCAT, lycat, agpat8, alcat1, 1agpat8, AGPATP8, 1AGPAT8, AGPAT8, ALCAT1, HSRG1849, UNQ1849, AI181996, Agpat8, Alcat1, Gm91, Lycat, RGD1565906
Epitope
Middle Region
(6), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Human
(8)
Host
Rabbit
(9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(9), (6), (6), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN502511
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Immunogen Synthetic peptide directed towards the middle region of human LYCAT
Sequence YLYSLVKWYF IITIVIFVLQ ERIFGGLEII ELACYRLLHK QPHLNSKKNE
Predicted Reactivity Human :100 %,   Mouse :90 %,   Pig :90 %,   Rat :90 %,  
Characteristics This is a rabbit polyclonal antibody against LYCAT. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name LYCAT
Background LYCAT is an acyl-CoA:lysocardiolipin acyltransferase. It possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. LYCAT recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. LYCAT acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. It converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. LYCAT is required for establishment of the hematopoietic and endothelial lineages.
Molecular Weight 44 kDa
Gene ID 253558
NCBI Accession NP_001002257, NM_001002257
UniProt B4DYR5
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 376 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Lysocardiolipin Acyltransferase 1 (LCLAT1) (Middle Region) antibody WB Suggested Anti-LYCAT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate
anti-Lysocardiolipin Acyltransferase 1 (LCLAT1) (Middle Region) antibody (2) Host: Rabbit
Target Name: LCLAT1
Sample Tissue: Human 721_B cell
Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5.0 µg/mL Lysate Quantity:.41 µg/lane/laneGel Concentration: 12 %
Background publications Wang, Faloon, Tan et al.: "Mouse lysocardiolipin acyltransferase controls the development of hematopoietic and endothelial lineages during in vitro embryonic stem-cell differentiation." in: Blood, Vol. 110, Issue 10, pp. 3601-9, 2007 (PubMed).

Validation Images
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