LCLAT1 antibody (Middle Region)

Catalog No. ABIN2781857

There is 1 publication for this product available. The Rabbit Polyclonal anti-LCLAT1 antibody is suitable to detect LCLAT1 in samples from Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse and Rat. It has been validated for WB.

Quick Overview for LCLAT1 antibody (Middle Region) (ABIN2781857)

Target: LCLAT1 (Lysocardiolipin Acyltransferase 1 (LCLAT1))

Reactivity: Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCLAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LCLAT1 Antibody

Binding Specificity: Middle Region

Purpose: LYCAT Antibody - middle region

Sequence: YLYSLVKWYF IITIVIFVLQ ERIFGGLEII ELACYRLLHK QPHLNSKKNE

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against LYCAT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LYCAT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44426-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-LCLAT1 antibody (ABIN2781857)

Zou, Synan, Li, Xiong, Manni, Liu, Chen, Zhao, Shiva, Tyurina, Jiang, Lee, Das, Ray, Ray, Kagan, Mallampalli: "LPS impairs oxygen utilization in epithelia by triggering degradation of the mitochondrial enzyme Alcat1." in: Journal of cell science, Vol. 129, Issue 1, pp. 51-64, (2016) (PubMed).

Target Details for LCLAT1

Target: LCLAT1 (Lysocardiolipin Acyltransferase 1 (LCLAT1))

Alternative Name: LYCAT

Background:

Background Information: LYCAT is an acyl-CoA:lysocardiolipin acyltransferase. It possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. LYCAT recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. LYCAT acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. It converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. LYCAT is required for establishment of the hematopoietic and endothelial lineages.

Gene Name: Lysocardiolipin acyltransferase 1

Alternative Symbols: LYCAT, AGPAT8, ALCAT1, 1AGPAT8, UNQ1849, HSRG1849

Protein Name: cDNA FLJ61482, highly similar to Homo sapiens lysocardiolipin acyltransferase (LYCAT), transcript variant 1, mRNA EMBL BAG63827.1

Molecular Weight: 44kDa

Gene ID: 253558

NCBI Accession: NP_001002257

UniProt: Q6UWP7