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His Tag antibody

Details for Product No. ABIN559683, Supplier: Login to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
Reactivity
Tag
479
22
7
3
Host
Mouse
270
180
31
26
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
50
38
35
15
12
8
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
430
273
165
75
63
32
32
13
12
11
8
6
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Sequence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Target Type Tag
Research Area Tags/Labels
Pathways
Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-His Tag antibody (ABIN559683) LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins i...
 image for anti-His Tag antibody (ABIN559683) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, pro...
 image for anti-His Tag antibody (ABIN559683) Standard ladder containing five different His-tagged proteins, untransfected control ...
Product cited in: Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).