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His Tag antibody

Details for Product No. ABIN559683, Supplier: Login to see New
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Synonyms AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI
(478), (22), (7), (3)
(269), (180), (31), (26), (3)
Clonality (Clone)
Monoclonal ()
(50), (38), (35), (15), (12), (8), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(429), (273), (164), (74), (63), (32), (32), (13), (12), (11), (8), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 1 to 2 Business Days
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Sequence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Target Type Tag
Research Area Tags/Labels
Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-His Tag antibody (ABIN559683) LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins i...
 image for anti-His Tag antibody (ABIN559683) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, pro...
 image for anti-His Tag antibody (ABIN559683) Standard ladder containing five different His-tagged proteins, untransfected control ...
Product cited in: Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Catalog No. ABIN559683
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