His Tag antibody

Details for Product No. ABIN559683
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms CaT2, Ecac1
Reactivity
Tag
(320), (28), (7), (4), (3), (2), (2), (1), (1)
Host
Mouse
(190), (130), (22), (19), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(36), (26), (23), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Dot Blot (DB), ELISA, Immunoprecipitation (IP), Immunostaining (IS), Western Blotting (WB)
(285), (163), (112), (57), (47), (30), (26), (20), (13), (10), (7), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
Catalog no. ABIN559683
Quantity 100 µg
Price
141.43 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 1 to 2 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Clone HIS-H8
Isotype IgG2b
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-His Tag antibody LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
anti-His Tag antibody (2) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)
anti-His Tag antibody (3) Standard ladder containing five different His-tagged proteins, untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
Product cited in: Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hosts (190), (130), (22), (19), (2)
Reactivities (320), (28), (7), (4), (3), (2), (2), (1), (1)
Applications (285), (163), (112), (57), (47), (30), (26), (20), (13), (10), (7), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Conjugates (36), (26), (23), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3)
back to top