Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN559683

His Tag antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CaT2, Ecac1
Reactivity
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Dot Blot (DB), ELISA, Immunoprecipitation (IP), Immunostaining (IS), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN559683
Quantity 100 µg
Price
141.43 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 1 to 2 Business Days
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Clone HIS-H8
Isotype IgG2b
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Preservative Sodium azide
Storage -20 °C
Product cited in: Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Alternatives for antigen "His Tag", type "Antibodies"
Hosts (172), (124), (23), (21), (3)
Reactivities (323), (9), (8), (3), (2), (2), (1), (1)
Applications (272), (164), (102), (51), (37), (30), (23), (20), (14), (10), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Conjugates (36), (24), (23), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon