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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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  • Target: His Tag
  • Primary
Catalog no. Product Reactivity Host / Clonality Application Price & lead time
ABIN387750
His Tag antibody Primary Antibody Human Mouse Monoclonal Clone 6AT18
  • WB (Western Blotting: Validation image available)
$451.00 View price
  • 200 μL
  • 50 μL
Delivery in 10 to 12 Business Days Shipping to: United States
ABIN1573881
His Tag antibody Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
$164.26 View price
  • 40 μg
  • 100 μg
  • 200 μg
Delivery in 2 to 4 Business Days Shipping to: United States
ABIN100492
Rockland Original
His Tag antibody Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • DB (Dot Blot: Listed application)
$345.00 View price
  • 100 μg
  • 25 μL
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN387699
His Tag antibody Primary Antibody – Mouse Monoclonal Clone 6G2A9
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • FACS (Flow Cytometry: Listed application)
$565.00 View price
  • 100 μg
Delivery in 4 to 7 Business Days Shipping to: United States
ABIN652278
His Tag antibody Primary Antibody Human Rabbit Polyclonal Clone RB19960
  • WB (Western Blotting: Validation image available)
$451.00 View price
  • 200 μL
  • 50 μL
Delivery in 10 to 12 Business Days Shipping to: United States
ABIN964552
Rockland Original
His Tag antibody Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • IP (Immunoprecipitation: Listed application)
$369.00 View price
  • 100 μg
  • 25 μL
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN7449520
His Tag antibody (HRP) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$440.15 View price
  • 100 μg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7131650
His Tag antibody Primary Antibody – Mouse Monoclonal Clone 3G5H8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
$235.40 View price
  • 100 μL
  • 50 μL
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN7795586
Recombinant His Tag antibody Primary Antibody – Rabbit Monoclonal Clone BLRE06M
  • WB (Western Blotting: Validation image available)
  • IP (Immunoprecipitation: Listed application)
$594.15 View price
  • 100 μL
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN6720637
Rockland Original
His Tag antibody (DyLight 649) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN964554
Rockland Original
His Tag antibody (HRP) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • IHC (Immunohistochemistry: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720721
Rockland Original
His Tag antibody (Cy3) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FACS (Flow Cytometry: Listed application)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 50 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720719
Rockland Original
His Tag antibody (DyLight 800) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN559683
His Tag antibody Primary Antibody – Mouse Monoclonal Clone HIS-H8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • DB (Dot Blot: Listed application)
  • ISt (Immunostaining: Listed application)
$169.72 View price
  • 100 μg
  • 50 μg
  • 200 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN7426805
His Tag antibody Primary Antibody Various Species Mouse Monoclonal Clone D10
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • ICC (Immunocytochemistry: Listed application)
  • IHC (fro) (Immunohistochemistry (Frozen Sections): Listed application)
  • CLIA (Chemiluminescence Immunoassay: Listed application)
$357.19 View price
  • 100 μL
  • 1 mL
  • 20 μL
  • 200 μL
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN2474014
His Tag antibody (Alkaline Phosphatase (AP)) Primary Antibody – Mouse Monoclonal Clone AD1-1-10
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$534.08 View price
  • 0.1 mg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN2474018
His Tag antibody (FITC) Primary Antibody – Mouse Monoclonal Clone AD1-1-10
  • IF (Immunofluorescence: Listed application)
  • FACS (Flow Cytometry: Listed application)
$517.08 View price
  • 0.1 mg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN2443717
His Tag antibody (C-Term, N-Term) Primary Antibody – Mouse Monoclonal Clone HIS-H8
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • DB (Dot Blot: Listed application)
$351.90 View price
  • 100 μg
  • 200 μg
Delivery in 21 to 27 Business Days Shipping to: United States
ABIN304882
His Tag antibody (FITC) Primary Antibody – Mouse Monoclonal Clone AD1-1-10
  • IHC (Immunohistochemistry: Listed application)
  • FACS (Flow Cytometry: Listed application)
$571.27 View price
  • 100 μg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN5668255
Recombinant His Tag antibody (N-Term) Primary Antibody – Mouse Monoclonal Clone 3D5
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
Primary Antibody
  • WB (Western Blotting: Validation image available)
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Clone
6AT18
Conjugate
unconjugated
Isotype
IgG1
Catalog No. ABIN387750
$451.00
Plus shipping costs $50.00
200 μL (More quantities available)
Shipping to: United States
Delivery in 10 to 12 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN1573881
$164.26
Plus shipping costs $50.00
40 μg
Shipping to: United States
Delivery in 2 to 4 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • DB (Dot Blot: Listed application)
Supplier Product No.
Rockland 600-401-382
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN100492
$345.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
6G2A9
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN387699
$565.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Clone
RB19960
Conjugate
unconjugated
Isotype
Ig Fraction
Catalog No. ABIN652278
$451.00
Plus shipping costs $50.00
200 μL (More quantities available)
Shipping to: United States
Delivery in 10 to 12 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • IP (Immunoprecipitation: Listed application)
Supplier Product No.
Rockland 200-301-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
unconjugated
Isotype
IgG1 kappa
Catalog No. ABIN964552
$369.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
HRP
Isotype
IgG
Catalog No. ABIN7449520
$440.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3G5H8
Conjugate
unconjugated
Isotype
IgG2a
Catalog No. ABIN7131650
$235.40
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • IP (Immunoprecipitation: Listed application)
Host
Rabbit
Clonality
Monoclonal
Clone
BLRE06M
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG
Catalog No. ABIN7795586
$594.15
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-343-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 649
Isotype
IgG1 kappa
Catalog No. ABIN6720637
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • IHC (Immunohistochemistry: Listed application)
Supplier Product No.
Rockland 200-303-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
HRP
Isotype
IgG1 kappa
Catalog No. ABIN964554
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FACS (Flow Cytometry: Listed application)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-304-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
Cy3
Isotype
IgG1 kappa
Catalog No. ABIN6720721
$490.00
Plus shipping costs $50.00
50 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • DB (Dot Blot: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-345-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 800
Isotype
IgG1 kappa
Catalog No. ABIN6720719
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • DB (Dot Blot: Listed application)
  • ISt (Immunostaining: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
HIS-H8
Conjugate
unconjugated
Isotype
IgG2b
Catalog No. ABIN559683
$169.72
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 3 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • ICC (Immunocytochemistry: Listed application)
  • IHC (fro) (Immunohistochemistry (Frozen Sections): Listed application)
  • CLIA (Chemiluminescence Immunoassay: Listed application)
Reactivity
Various Species
Host
Mouse
Clonality
Monoclonal
Clone
D10
Conjugate
unconjugated
Isotype
IgG1 kappa
Catalog No. ABIN7426805
$357.19
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
AD1-1-10
Conjugate
Alkaline Phosphatase (AP)
Isotype
IgG1
Catalog No. ABIN2474014
$534.08
Plus shipping costs $50.00
0.1 mg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • IF (Immunofluorescence: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
AD1-1-10
Conjugate
FITC
Isotype
IgG1
Catalog No. ABIN2474018
$517.08
Plus shipping costs $50.00
0.1 mg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • DB (Dot Blot: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
HIS-H8
Conjugate
unconjugated
Isotype
IgG2b
Binding Specificity
C-Term, N-Term
Catalog No. ABIN2443717
$351.90
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 21 to 27 Business Days
Primary Antibody
  • IHC (Immunohistochemistry: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
AD1-1-10
Conjugate
FITC
Isotype
IgG1
Catalog No. ABIN304882
$571.27
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG1, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668255
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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