His Tag Antibodies
(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays.
Here are some common applications of antibodies that detect His tags:
- Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
- Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.
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His Tag Antibodies by Grade
Find His Tag Antibodies with a specific Grade. The Grade listed below are among those available. Click on a link to go to the corresponding products.
His Tag Antibodies by Reactivity
Find His Tag Antibodies for a variety of species such as anti-Tag His Tag, anti-Human His Tag, anti-Various Species His Tag. The species listed below are among those available. Click on a link to go to the corresponding products.
His Tag Antibodies by Host
Find His Tag Antibodies with a specific Host. The Host listed below are among those available. Click on a link to go to the corresponding products.
His Tag Antibodies by Binding Specificity
Find His Tag Antibodies with a specific epitope. The epitopes listed below are among those available. Click on a link to go to the corresponding products.
His Tag Antibodies by Clonality
Find available monoclonal or polyclonal His Tag Antibodies. Click on a link to go to the corresponding products.
Popular His Tag Antibodies
Product
Reactivity
Application
Validations
Cat. No.
Quantity
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Reactivity
Application
WB, ELISA, IP, IHC
Validations
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Cat. No.
ABIN100492
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Human
Application
WB
Validations
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- (3)
Cat. No.
ABIN387750
Quantity
200 μL
Datasheet
Datasheet
Reactivity
Application
WB, ELISA
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- (3)
Cat. No.
ABIN1573881
Quantity
40 μg
Datasheet
Datasheet
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Application
WB, ELISA, IP, IHC, FACS
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- (4)
Cat. No.
ABIN964552
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Application
WB, ELISA, IP, IF, FACS
Validations
- (30)
- (3)
Cat. No.
ABIN387699
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Human, Tag
Application
WB
Validations
- (16)
- (2)
Cat. No.
ABIN722438
Quantity
100 μL
Datasheet
Datasheet
Reactivity
Application
WB, ELISA, IP
Validations
- (1)
- (3)
Cat. No.
ABIN964554
Quantity
100 μg
Datasheet
Datasheet
Reactivity
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Application
WB
Validations
- (1)
- (2)
Cat. No.
ABIN652278
Quantity
200 μL
Datasheet
Datasheet
Reactivity
Application
WB, ELISA
Validations
- (3)
Cat. No.
ABIN294958
Quantity
40 μg
Datasheet
Datasheet
Reactivity
Application
WB, ELISA, IP
Validations
- (1)
- (2)
Cat. No.
ABIN964555
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Application
WB, ELISA, IP, FACS, ICC, IHC (p)
Validations
- (3)
Cat. No.
ABIN5579852
Quantity
50 μg
Datasheet
Datasheet
Reactivity
Application
WB, IP, FACS, ICC, IHC (p)
Validations
- (3)
Cat. No.
ABIN5579851
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Application
WB, ELISA, IP, DB, ISt
Validations
- (5)
- (3)
Cat. No.
ABIN559683
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Application
WB, FM, FLISA
Validations
- (2)
- (3)
Cat. No.
ABIN6720637
Quantity
100 μg
Datasheet
Datasheet
Reactivity
Application
WB, ELISA
Validations
- (2)
Cat. No.
ABIN7131650
Quantity
100 μL
Datasheet
Datasheet
Latest Publications for our His Tag Antibodies
: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).
: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).
: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).
: "Intellectual disability-associated UNC80 mutations reveal inter-subunit interaction and dendritic function of the NALCN channel complex." in: Nature communications, Vol. 11, Issue 1, pp. 3351, (2020) (PubMed).
: "Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain." in: Acta neuropathologica communications, Vol. 6, Issue 1, pp. 25, (2019) (PubMed).
: "Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist." in: The Journal of clinical investigation, Vol. 129, Issue 4, pp. 1713-1726, (2019) (PubMed).
: "B-cell depletion induces a shift in self antigen specific B-cell repertoire and cytokine pattern in patients with bullous pemphigoid." in: Scientific reports, Vol. 9, Issue 1, pp. 3525, (2019) (PubMed).
: "Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia." in: Infection and immunity, Vol. 87, Issue 6, (2019) (PubMed).
: "HIV Infection Induces Extracellular Cathepsin B Uptake and Damage to Neurons." in: Scientific reports, Vol. 9, Issue 1, pp. 8006, (2019) (PubMed).
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